Faculty of Physics - University of Bucharest

UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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2024-11-22 1:52

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Conference: Bucharest University Faculty of Physics 2013 Meeting

Section: Optics, Spectroscopy, Plasma and Lasers

Title:

Laser processing of biodegradable calcium phosphates/ poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanocomposite thin films by Matrix Assisted Pulsed Laser Evaporation

Authors:

C.Nita1(5), G. Socol(1), L. Sima(3), L. Radulescu (4), F. Sima1, M. Socol(5), E. Axente1, V. Grumezescu(1), R. Cristescu(1), M. Miroiu(1), F. Antohe(4), C.S. Breazu(5), A. Stanculescu(5), I. Zgura(5), M. Chiritoiu(3)

Affiliation:

(1) National Institute for Lasers, Plasma and Radiation Physics, Magurele, Ilfov, Romania

(2)Faculty of Physics, University of Bucharest,Romania

3. Department of Molecular Cell Biology, Institute of Biochemistry, Romanian Academy, 296 Splaiul Independentei, 060031, Bucharest 17, Romania

4. Institute of Cellular Biology and Pathology N. Simionescu/Bucharest/Romania

5. National Institute of Materials Physics, Magurele, Ilfov, Romania

E-mail

cristina.nita@inflpr.ro

Keywords:

MAPLE, Thin films, biopolymers

Abstract:

In the last decade a considerable amount of work has been focused on medical and pharmaceutical research, especially on the development of new improved drug systems such as controlled drug delivery and its applications for tissue engineering for support and stimulation of tissue growth. In our experiments, we use Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) which is known like a biocompatible polymer completely biodegradable, with degradation products natural metabolites in human blood. The calcium phosphates (among them, HA and β-TCP) are the most important constituents of vertebrates` hard tissues (bones, teeth, tendons), to which they render stability, hardness and functionality.We report on the deposition of lysozyme embedded in nanocomposite polymeric coatings on grade 4 CP titanium substrates by Matrix Assisted Pulsed Laser Evaporation (MAPLE) and Dip Coating (DC) techniques. Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique appeared as response to the necessity to deposition high quality thin films from substances with complex chemical composition and functionally very fragile. This technique offers a “delicate” transfer mechanism which allow to extend the applicability interval to the compounds with big molecular weight or sensitive at high temperature. The physico-chemical properties of the coatings were investigated by Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy and X-Ray Difraction. We proved that the addition of β-tricalcium phosphates (β-TCP) or hydroxylapatite (HA) into different PHBV polymeric matrixes induces enhanced bioactivity compared with simple PHBV coatings. Moreover, we found out an improvement of mechanical properties and hydrophilicity. FTIR spectra of the thin films were found to be highly similar to the spectrum of the dropcast. Additional investigations, by scanning electron microscopy evidenced a typical morphology after MAPLE transfer. The results showed the decrease of nanocomposite biodegradability with the increasing of calcium phosphate amount. The significant results entitle us to continue the further studies and put in evidence the fact that chosen biopolymers are promising candidates for the aimed application.