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Conference: Bucharest University Faculty of Physics 2009 Meeting

Section: Biophysics; Medical Physics

Title:

Differences between the proteic expression of ASIC-1, -2, and -3 in diabetic neuropathy

Authors:

Beatrice Mihaela Radu (1), Mihai Radu (2), Diana Rotaru (1-3), Adina Daniela Iancu (1-4), Dorel Lucian Radu (4)

Affiliation:

(1)Department of Animal Physiology and Biophysics, Center of Neurobiology and Molecular Physiology, Faculty of Biology, University of Bucharest, Splaiul Independentei, 91-95, Bucharest, 050095, Romania

(2) Department of Health and Environmental Physics, ‘Horia Hulubei’ National Institute for Physics and Nuclear Engineering, Atomistilor, 407, Măgurele, 077125, Romania

(3) National Sanitary-Veterinary and Food Safety Authority, General Food Safety Directorate, Food Quality, Standards and Marks, Food Quality Service, Street Negustori, 1B, District 2, Bucharest, 023951, Romania

(4) National Institute for Microbiology and Immunology ‚Cantacuzino’, Laboratory of Cellular Immunity, Splaiul Independentei, 103, Bucharest, 050096, Romania

E-mail

beatrice_macri@yahoo.com

Keywords:

ASIC, sensory neurons, immunofluorescence

Abstract:

Diabetic neuropathies are a family of nerve disorders caused by diabetes. Nerve damage is likely due to a combination of factors: metabolic factors, neurovascular factors, autoimmune factors, mechanical injury to nerves, inherited traits that increase susceptibility to nerve disease and life style factors (smoking or alcohol). Peripheral neuropathy, the most common type of diabetic neuropathy, causes pain or loss of feeling in the toes, feet, legs, hands, and arms. In our study, we have studied the diabetic neuropathy syndrome developed after 10 weeks of persistent hyperglycemia in sensory neurons from TCR-HA+/-/Ins-HA+/- mice. By means of immunoflorescence, the changes in the level of expression of acid sensing ion channels (ASIC) has been analysed in detail. Only the levels of expression of ASIC1 and ASIC3 are increased in TCR-HA+/-/Ins-HA+/- mice compared to Balb/c mice. No significant change is detected in the expression of ASIC2. These effects might be explained by the fact that in type I diabetes, most of the receptors are glycosilated due to the increased blood glucose levels. These preliminary data are very important for future pharmacological tests for the efficiency of the anti-diabetics. This study was financed by the grant PNCDI2 41-074/2007.