| |
 |
UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-21 21:07 |
 |
|
|
|
Conference: Bucharest University Faculty of Physics 2008 Meeting
Section: Optics, Spectroscopy, Plasma and Lasers
Title:
Preparation of surfaces of polymeric materials
for interaction with biological cells
Authors:
E.C. Stancu1, M.D. Ionita1, M. Teodorescu1, G. Dinescu1, L. M. Stefan2, L. Moldovan2
Affiliation:
1National Institute for Laser, Plasma and Radiation Physics, Magurele MG-16, 077125 Magurele Bucharest, Romania
2National Institute of Research and Development for Biological Sciences, 296th Independence Spl, sector 6, Bucharest, Romania
E-mail
dinescug@infim.ro
Keywords:
atmospheric pressure plasma, surface modification, cell adhesion
Abstract:
The need of improving cell / surface interaction has introduced plasma techniques in the field of biomaterials. The treatments of polymers surface are performed using atmospheric pressure plasma, as an interesting alternative of low pressure plasma.
The development of a new technique based atmospheric pressure technique suitable for treating polymer surfaces without damage and the test of these surfaces with regard to cellular responses and cell adhesion was the aim of this study. As support for the experiments, thin foils of polymers were used.
We describe a process that uses a cold plasma source jet based on a radiofrequency discharge and working in Ar in free atmosphere. In order to improve the biocompatibility of the surface, the process can be followed by immersion into monomers solution or by exposure to monomers vapour, for example acrylic acid, ethylenediamine, solution leading to grafting of these molecules on the treated surfaces. It is known that surfaces containing groups like carboxyl and amine generally present different cell adhesion properties.
The ability of plasma in producing reactive surfaces was measured by contact angle and scotch tape adhesion tests. Reference samples (untreated), plasma treated samples and grafted samples are under tests of interaction with osteoblasts. Efforts are in course to individuate the effect of each individual group on cell growth and morphology.
|
|
|
|