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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 1:37 |
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Conference: Bucharest University Faculty of Physics 2006 Meeting
Section: Electricity and Biophysics
Title:
Electrophysiological characteristics of the neurons from dorsal root ganglia upon superfusion of coumarines
Authors:
Beatrice Macri(1), Adina Iancu (1)(2), Maria-Luiza Flonta (1)
Affiliation:
(1) Faculty of Biology, Department of Animal Physiology and Biophysics, Splaiul Independentei, 91-95, Bucharest, R-050095
(2) National Institute R&D of Microbiology and Immunology "Cantacuzino", Splaiul Independentei 103, Bucharest, R-050096
E-mail
beatrice_macri@yahoo.com
Keywords:
coumarin, coumarin 3-carboxylic acid, ICa(L), primary neuronal culture
Abstract:
Coumarin (1,2-Benzopyrone or 2H-1-Benzopyran-2-one) is a chemical compound found in many plants (such as tonka bean, woodruff and bison grass) and it has an important clinical value, being the precursor for several anticoagulants, notably warfarin. One of its synthetic derivates is coumarin 3-carboxylic acid. Beside there anticoagulant action, the pharmacological spectrum of coumarins is far more complex. Our study aims to elucidate the effects of these drugs on neurons from dorsal root ganglia. Using patch-clamp technique, in the whole-cell configuration, several electrophysiological characteristics of the adult rat neurons were analyzed in the absence or presence of coumarins (0.1-100 microM). Thus, a complete suppression of the Na+ current and a 10 fold reduction of the amplitude for the Ca(L) current was recorded upon perfusion of coumarins (100 microM). Ih currents were insensitive to coumarins application. A mixed population of neurons is present in dorsal root ganglia, and their Ca2+ currents are quite heterogeneous, that implies further investigation of there kinetics in the presence of coumarins. These results are in agreement with the effect of 0.3-100 microM osthole (a coumarin from Cnidium monnieri) on voltage-dependent L-type Ca2+ current in mouse neuroblastoma NG108-15 cells.
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