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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 1:30 |
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Conference: Bucharest University Faculty of Physics 2001 Meeting
Section: Optics and Spectroscopy
Title:
Spectroscopic study of cytostatics
Authors:
M.L. Pascu, S.Truica, A.Staicu, L.Voicu, D.Gazdaru*, I.Mogos**
Affiliation:
The National Institute for Lasers, Plasma and Radiation Physics,
Lasers Department, P.O. Box MG 36, 76900, Bucharest, Romania
* University of Bucharest, Faculty of Physics, Biophysics Department,
Bucharest
** Oncology Institute "Prof. Dr. Alexandru Trestioreanu, Sos. Fundeni
252, Bucharest
E-mail
Keywords:
Abstract:
A spectroscopic study for the characterisation of methotrexate,
cytostatic drug used frequently in cancer therapy, was reported.
The absorption and fluorescence (emission and excitation) spectra
were measured for methotrexate solutions in natural saline and sodium
hydroxide at concentration in the range 10-5 - 10-6 M and pH 8.4. The
absorption spectrum exhibits spectral bands in the range 250nm - 450nm.
The fluorescence excitation was made at 340nm and 370nm, the fluorescence
emission was detected in the spectral range 400nm - 500nm with a maximum
at 470nm.
The effects of UV irradiation on spectroscopic characteristics of
methotrexate were investigated. The irradiation was realised using a UV
classic lamp with Xe and emission in spectral range 325nm - 420nm.. The
power density was 11mW/cm2 and the exposure time varied between 15 and 195
minutes.
Major modifications on absorption bands for UV irradiation times
longer than 1 hour were observed. The methotrexate solutions become
strongly fluorescent by UV irradiation. The detected changes indicate that
a photochemical process involving MTX takes place. Further investigations
to elucidate these processes are in progress.
The present studies are indicated possible application in clinical
chemotherapy treatment procedure. This kind of characterisation will be
extended to a large series of cytostatics.
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