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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-25 0:00 |
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Conference: Bucharest University Faculty of Physics 2012 Meeting
Section: Biophysics; Medical Physics
Title:
Methods and specific markers of differentiation of mouse osteoblasts in culture
Authors:
Ancuta Elena BACIU (1), Dana Alice MANDA (2), A. POPESCU (1)
Affiliation:
(1) University of Bucharest, Faculty of Physics, P.O.BOX MG-11, Magurele, Romania
(2)C.I. Parhon National Institute of Endocrinology, Bucharest, Romania
E-mail
ancutaelenabaciu@yahoo.com
Keywords:
Osteoblasts, bone mineralization process, dexamethazone
Abstract:
Osteoblasts, the bone forming cells, are derived from mesenchymal stem cells following several stages of differentiation from the pre - osteoblasts cells to mature osteoblasts cells.The objective of this work is to test several methods for obtaining mouse osteoblasts and their differentiation in culture. The experimental protocol that was obtained is then tested for the biological study of osteoblasts. We obtained a viable culture, studied in five steps in which the cells were progressively differentiated until the final phase of bone matrix. A typical cell growth curve with a log phase between the step 1 and step 4 was obtained. This experimental protocol has been validated by applying a treatment with dexamethazone (DXM) using different DXM concentrations. The dose-response curve showed that DXM significantly inhibited alkaline phosphatase activity. This protocol was forward applied for the study of the effect of calcium fructoborate on bone mineralization process.
We have obtained a viable and functional experimental model that is characterized by specific markers for each stage of osteoblast differentiation. The development of effective therapies for diseases affecting bone formation depends directly on understanding the mechanism regulating bone remodeling.
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