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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 1:59 |
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Conference: Bucharest University Faculty of Physics 2012 Meeting
Section: Biophysics; Medical Physics
Title:
A comparative study of the antioxidant properties of extracts obtained from blueberry and blackcurrant leaves on the cell membranes damage
Authors:
Diana GOLEA (1,2), Steliana RODINO (2), Carmen MANOLE (2), Laura TUGULEA (1), Alina BUTU (2)
Affiliation:
(1) Department of Electricity, Solid State and Biophysics, Faculty of Physics, University of Bucharest, Str. Atomistilor 405, 077125, Bucharest-Magurele, Romania
(2) National Institute of Research and Development for Biological Sciences, Splaiul Independenţei 296, district 6, 060031, Bucharest, Romania
E-mail
goleadiana@yahoo.com
Keywords:
antioxidants, lipid oxidation, blueberry, blackcurrant
Abstract:
The membranar lipids are a major target of the aggression of free radicals due to the presence of double bond in polyunsaturated fatty acid structure. The lipid oxidation processes follow already identified fundamental mechanisms, in foods or biological tissues things may be more complicated. The subject of the present research was to investigate, by comparison, the antioxidant properties of two extracts obtained from blueberry (Vaccinium mystillus) and blackcurrant (Ribes nigrum) leaves on membrane lipids oxidation due to the free radical attack. First, the hydroalcoholic extracts were obtained by sonication method and cold preparation in order to preserve the properties of the plants. Then, the extracts were characterized by determination of total flavonoids concentration and polyphenol carboxylic acids concentration. The studies were performed using liposomes which were prepared by thin lipid film hydration method and used as artificial membrane models, mimicking cell membranes. The antioxidant behaviour of extracts was investigated using UV-VIS absorption and emission spectroscopy. After testing the antioxidant activity for 5 hours it was demonstrated that lipids are much better protected against oxidation when the blueberry leaves extract was used as compared with blackcurrant leaves extract.
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