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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 1:21 |
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Conference: Bucharest University Faculty of Physics 2012 Meeting
Section: Biophysics; Medical Physics
Title:
Evaluation of electrochemical enzyme based-biosensors used in heavy metal ions detection
Authors:
Melinda DAVID (1), Monica FLORESCU (2) , Laura TUGULEA (1)
Affiliation:
(1)Faculty of Physics, University of Bucharest, Bucharest, Romania
(2) Faculty of Medicine, Transilvania University of Brasov, Brasov, Romania
E-mail
melinda.dav@gmail.com
Keywords:
biosensors, screen-printed electrodes, heavy metals, acetylcholinesterase, mediators, sol-gel
Abstract:
Biosensor attributes which permanently need to be optimized are response time, initial and long term activity of the biological component, analytical range, sensitivity, selectivity, detection limit and reproducibility. The biosensor stability and analytical performance depends on the matrix and the immobilisation process of biological components used. In this work we have used mediated carbon-ink screen-printed electrodes for enzyme-based biosensors development used as detection tool for heavy metal ions through enzymatic inhibition. Two immobilization methods were used for acetylcholinesterase (AChE) immobilization on electrode surface together with two electrochemical mediators. The methods used for the encapsulation of the enzyme were: cross-linking with bi-functional reagents like glutar-aldehyde (GA) and encapsulation with sol-gel method. The used mediators were TCNQ (tetracianoquinodimethan) and CoPC (cobalt phthalocyanine). Comparison between biosensors performance was made function of electrolyte pH, especially for more acidic values were heavy metals are present in ionic form. The working potential window and background currents were evaluated by cyclic voltammetry and amperometry.
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