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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-23 17:44 |
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Conference: Bucharest University Faculty of Physics 2009 Meeting
Section: Biophysics; Medical Physics
Title:
Synaptic plasticity by optical guidance
Authors:
Daniel Dumitru Banciu (1), Beatrice Mihaela Radu(1), Mihai Radu (2), Adela Marin (1), Tudor Savopol (3)
Affiliation:
(1)Department of Animal Physiology and Biophysics, Center of Neurobiology and Molecular Physiology, Faculty of Biology, University of Bucharest, Splaiul Independentei, 91-95, Bucharest, 050095, Romania
(2) Department of Health and Environmental Physics, ‘Horia Hulubei’ National Institute for Physics and Nuclear Engineering, Atomistilor, 407, Măgurele, 077125, Romania
(3) UMF `Carol Davila, Bvd Eroilor Sanitari, 8, Bucharest, Romania
E-mail
bddbdd@yahoo.com
Keywords:
synaptic plasticity, optical tweezers
Abstract:
Control over neuronal growth is a fundamental objective in neuroscience, cell biology, developmental biology, biophysics, and biomedicine and is particularly important for the formation of neural circuits in vitro, as well as nerve regeneration in vivo. Adult Wistar rats (150-200)g were used in order to prelevate the dorsal root ganglia. Dissociated sensory neurons were grown in different conditions (with and without inhibitors for the glial cell proliferation). We have shown experimentally that we can use weak optical forces to guide the direction taken by the leading edge, or growth cone, of a sensory nerve cell. Fluorescence measurements by Ca2+ imaging were done in order to test the synaptic formation.
This study was financed by grant PNCDI2 61-011/2007.
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