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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-24 9:18 |
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Conference: Bucharest University Faculty of Physics 2011 Meeting
Section: Biophysics; Medical Physics
Title:
The biological effects of simulated magnetic contamination of plant seedlings during their early ontogenetic stages
Authors:
Astefanoaei Corina, Racuciu Mihaela*, Creanga Dorina
Affiliation:
Univ. Al. I. Cuza, Physics Department, Iasi, Romania
*Univ. L. Blaga, Faculty of Sciences, Sibiu, Romania
E-mail
corina_astefanoaei@yahoo.com
Keywords:
environmental toxicity, magnetic constraints, agrotechnical plants
Abstract:
The experiment aimed to evidence the changes induced by environment magnetic contamination on the photosynthesis pigments (chlorophyll a, chlorophyll b, carotene like pigments). Magnetic contamination was accomplished by the plant seedling supply with colloidal magnetic nanoparticles provided by diluted ferrofluid (10 and 100 μl/l). The biocompatible ferrofluid was prepared based on magnetite nanoparticles coated with tartaric acid and suspended in deionized water. Second plant sample array were exposed to magnetic field gradient (permanent magnets with central value of magnetic induction of 6 and 10 mT) while third plant array was simultaneously exposed to both magnetic constraints considering the possibility of their simultaneous actions in the natural environment. Biological material was consistent with Helianthus annuus (sunflower) caryopses with uniform genophond, germinated and grown in controlled environmental conditions (temperature, humidity, and lightning) in INCUCELL room. The exposure to the magnetic constraints lasted during the first seven days of their early ontogenesis. The biological influence was assessed by means of biochemical parameters related to the photosynthesis pigment contents assayed by applying Meyer-Bertenrath method and Lichtenhalter’s calculation formulae. The most significant changes (statistically assessed by Student t-test) were recorded at the level of photosynthesis efficiency in the plantlets grown with magnetic nanoparticles (80% diminution of the ratio chlorophyll a/ chlorophyll b, taken as indirect indicator of photosynthesis efficiency) and/or following the exposure to magnetic field (40% and respectively 70% diminution). In the same time the biosynthesis of secondary pigments (carotenes) appears to be stimulated –2-3 times increase of total carotene content. The main conclusion is related to the sensitivity of young plant seedlings to the environmental factors of magnetic nature as resulted from the study of photosynthesis pigments located into the tylakoidal membranes of chloroplasts.
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