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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 2:18 |
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Conference: Bucharest University Faculty of Physics 2010 Meeting
Section: Biophysics and Medical Physics;Electricity and Magnetism
Title:
Biophysical characteristics of TRPV1 receptor in type I autoimmune diabetes
Authors:
Beatrice Mihaela Radu(1), Adina Daniela Iancu (1, 2), Dorel Lucian Radu (2), Mihai Radu (3)
Affiliation:
(1) Department of Animal Physiology and Biophysics, Center of Neurobiology and Molecular Physiology, Faculty of Biology, University of Bucharest, Splaiul Independentei, 91-95, Bucharest, 050095, Romania
(2) `Cantacuzino’ National Institute for Microbiology and Immunology, Laboratory of Cellular Immunity, Splaiul Independentei, 103, Bucharest, 050096, Romania
(3) Department of Health and Environmental Physics, ‘Horia Hulubei’ National Institute for Physics and Nuclear Engineering, Atomistilor, 407, Măgurele, 077125, Romania
E-mail
beatrice_macri@yahoo.com
Keywords:
vanilloid receptor, capsaicin, patch-clamp, immunofluorescence microscopy, autoimmune diabetes
Abstract:
Transient receptor potential vanilloid (TRPV1) receptors are located on peripheral nerve fibers and known to function as a molecular integrator of different painful stimuli.
Whole-cell patch-clamp recordings were performed on primary cultures of sensory neurons from dorsal root ganglia (DRG) prelevated from type I diabetic TRC-HA+/-/Ins-HA+/- mice and Balb/c mice.
Peak current responses to 1 μM capsaicin were significantly larger (987 ± 112 pA, n = 30 cells from 5 mice) (p < 0.05) from DRG obtained from TRC-HA+/-/Ins-HA+/- mice as compared to the age matched Balb/c mice (550 ± 76 pA, n = 30 cells from 5 mice). These results suggest that altered TRPV1 currents correlate with altered thermal pain sensitivities in diabetic mice.
Expression of TRPV1 was significantly higher in the sensory neurons from diabetic mice (56 ± 2.3 gray value, n = 20 images from 5 mice, p < 0.05) as compared to age matched non-diabetic mice (45 ± 2.1, n = 20 images from 5 mice).
The enhanced functioning of TRPV1 receptors in DRG neurons, probably due to phosphorylation, oligomerisation or reallocation of these receptors on the cell surface, may contribute to hyperalgesia in early stages of diabetic neuropathy.
This study was financed by PNCDI2 41-074/2007
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