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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 2:12 |
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Conference: Bucharest University Faculty of Physics 2004 Meeting
Section: Electricity and Biophysics
Title:
Procaine Effect on Liposomes with Chlorophylla
Authors:
Laura Tugulea, Marcela Bărbîntă – Pătrascu, Mioara Petrus
Affiliation:
Faculty of Physics- University of Bucharest
P.O.Box MG-11, Bucharest-Magurele
E-mail
ltugulea@fpce1.fizica.unibuc.ro
Keywords:
liposomes, chlorphyll a, procaine, tocopherol, absorption and emission spectroscopy
Abstract:
The liposomes with chlorophylla are excellent model membranes and are successfully used to study the effect of different drugs on membranes. The spectroscopic properties of chlorophylla allow its use as sensor for the interactions at molecular level.
Unilamellar liposomes were obtained from multilamellar vesicles by sonication. The multilamellar vesicles were prepared using Chla/lipid films with specific molar ratio (lipid/Chla = 1/100) and different lipids. The effect of procaine was studied on Chla-liposomes and on Chla-liposomes containing also tocopherol incorporated in the bilayer. The drug was added in different concentrations to the suspension of liposomes.
The effect of procaine on Chla-liposomes was investigated by absorption and emission spectroscopy. Spectral criteria were developed in order to monitor the size of liposomes, the oxidation state of the components and for studying the effect of drug and the antioxidant effect of tocopherol. Chlorophylla and procaine proved to be competitors in the lipid bilayer and a dependence upon the lipid nature of procaine effects was evidentiated. Tocopherol effect is also dependent on lipid nature.
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