UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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Conference: Bucharest University Faculty of Physics 2013 Meeting


Section: Optics, Spectroscopy, Plasma and Lasers


Title:
Chitosan polymer structuring using laser methods for biomedical applications


Authors:
L. Rusen1(3), V. Dinca(1), C. Mustaciosu(2), B. Mitu(1), M. Filipescu(1), C. Luculescu(1), M. Zamfirescu(1), S. Sandel(1)


Affiliation:
1. INFLPR, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Str., 077125 Magurele, Bucharest, Romania

2. Horia Hulubei National Institute of Physics and Nuclear Engineering - IFIN HH, 30 Reactorului Str., Magurele, Bucharest, Romania

3 Physics Faculty, University of Bucharest, 405 Atomistilor Str., 077125 Magurele, Bucharest, Romania


E-mail
laurentiu.rusen@inflpr.ro


Keywords:
topography control, laser induced surface-modification, biopolymers, chitosan, SEM, AFM.


Abstract:
Laser based methods were used in order to induce different surface topographies in chitosan based thin films. Chitosan is a linear polysaccharide composed of randomly distributed β-(1-4)-linked D-glucosamine (deacetylated unit) and N-acetyl-D-glucosamine (acetylated unit). It is derived from chitin, one of the world most important natural polymers [1]. Chitosan, which is soluble in acidic aqueous media, it will maintain its structure in a neutral environment being an important tool in biomedical and pharmaceutical applications [2]. Moreover, chitosan is an appropriate choice for biological aplications due to its similarity to glycosaminoglycans, its biodegradability and nontoxicity. In this study, chitosan based structures were obtained by direct irradiation in air with different lasers (femtosecond laser: Ti Saphire-775 nm and excimer lasers: ArF(193 nm) and KrF (248 nm). To improve its biocompatibility and enhance the cellular attachment to chitosan-coated surfaces, other components, such as bioactive factors (i.e. Collagen, laminin) were added to the polymer. Morphological characteristics of the chitosan-based films surfaces were characterized by scanning electron microscopy and atomic force microscopy. From our preliminary in vitro studies, it was observed that the early cell growth was conditioned by the micro-topography of the chitosan surfaces. 1 Sunil A. Agnihotri, Nadagouda N. Mallikarjuna and Tejraj M. Aminabhavi (2004). "Recent advances on chitosan-based micro- and nanoparticles in drug delivery", Journal of Controlled Release 100 (1): 5–28. PMID 15491807 2 Marguerite Rinaudo, “Chitin and chitosan: Properties and applications”, Progress in Polymer Science, Volume 31, Issue 7, July 2006, Pages 603–632