| |
 |
UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 2:32 |
 |
|
|
|
Conference: Bucharest University Faculty of Physics 2005 Meeting
Section: Electricity and Biophysics
Title:
Biophysical properties of ASIC1a channels
Authors:
Beatrice Macri, Emil Neaga, Bogdan Amuzescu, Corina Prica, Maria-Luiza Flonta
Affiliation:
Department of Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, Splaiul Independentei, 91-95
E-mail
beatrice_macri@yahoo.com
Keywords:
ASIC1a channel, patch-clamp, amplitude decrease function
Abstract:
Acid-sensing ion channels (ASICs) represent a recently discovered group of ion channels belonging to the Deg/ENaC family ASIC1a is endogenously expressed in HEK293 cells. The goal of our study was to describe to mathematical function of amplitude decrease after repeated pH stimuli. We performed patch-clamp recording in whole-cell configuration, at –70 mV, and we applied two pH stimuli, 5.3 and 6.5. Recordings were done in the first day of cell culture and in the second day, significant differences of amplitude peak variation being noticed. The presence of ATP and GTP into the pipette solution also determined the modification of the amplitude variation profile. In conclusion, at very acidic pH (5.3) the amplitude follows an exponential decrease profile, and at medium acidic pH (6.5) it follows a linear decrease. At pH 5.3, the presence of ATP-GTP into the patch pipette modifies the tau of the exponential. Acidic stress in ischemia represent an important clinical issue and these data are important in drug design of efficient ligands against ASIC1a channels.
|
|
|
|