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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 2:01 |
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Conference: Bucharest University Faculty of Physics 2013 Meeting
Section: Biophysics; Medical Physics
Title:
Characterization of some physico-chemical
properties of cRGD peptide
Authors:
Ioana Eşanu (1,3), Claudia Chilom (1), Dana Niculae (3), Doina Găzdaru (1), Alexandru Nistorescu (1,2), Aurel Popescu (1)
Affiliation:
(1)Department of Electricity and Solid State and Biophysics, Faculty of Physics, University of Bucharest, Romania
(2)Space Applications for Human Health and Safety, Institute of Space Science, legal subsidiary of the National Institute for Laser, Plasma and Radiation Physics, Romania
(3)Radioisotopes and Radiation Metrology Department – Radiopharmaceuticals Research Centre, National Institute for Physics and Nuclear Engineering “Horia Hulubei”, Romania
E-mail
Keywords:
RGD peptides,fluorescence,Raman spectroscopy
Abstract:
RGD peptides are new tools for drug therapy and imaging of tumors, thrombosis and inflammatory-related diseases. P H-Glu[cyclo (Arg-Gly-Asp-D-Tyr-Lys)]2 is a bicyclic RGD peptide, recently reported to possess high affinity for αvβ3 integrin and another. In order to characterize the physico-chemical properties of the cRGD peptide, we had performed steady state fluorescence, Raman spectroscopy and differential scanning calorimetry. Raman spectra put in evidence the amide bands in the peptide and the existence of a small secondary structure of the peptide. Fluorescence experiments had showed the thermal denaturation of the peptide (between 25 °C and 80 °C), with a completely denaturated chain around 75 °C. pH dependence of the peptide shows great variation of the absorbance with pH values. Differential scanning calorimetry confirms the spectroscopic experiments, showing a 32 °C melting temperature (where half of the peptide chain is denaturated) and a 79 °C, at which all the peptidic chain is completely denaturated.
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