UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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Conference: Bucharest University Faculty of Physics 2002 Meeting


Section: Optics, Spectroscopy, Plasma and Lasers


Title:
Spectral and chemical caracterisation of pyrimidines cytostatics used as photosensiting materials in photodynamic therapy


Authors:
M.L. Pascu*, S. Truica* , A. Staicu*, L.Voicu*, D. Gazdaru**


Affiliation:
* The National Institut for Laser, Plasma and Radiation Physics, Laser Department, Bucharest, Romania.

**University of Bucharest, Faculty ofPhysics, Biophysics Departmant, Bucharest.


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Keywords:


Abstract:
Introduction of the photosensitizer properties of cytostatics drus was made, pointing out the fact that besides the biochemical action of the cytostatics their effects could be enhanced by the exposure to ligth at different doses. A spectroscopical characterisation of methotrexate and fluorouracil, cytostatic drugs used frequently in cancer therapy was performed. The absorption, emission and excitation spectra were measured for methotrexate solutions in natural saline and sodium hydroxide at concentration in the range 10-5 -10-6M and pH 8.4. The absorption, emission and excitation spectra were measured for fluorouracil solutions in natural saline at concentration in the range 10-4 -10-5M. The absorption spectrum exhibits spectral bands in the range 250nm - 450nm for both drugs. The fluorescence excitation for methotrexate was made at 340nm and 370nm, the fluorescence emission was detected in the spectral range 400nm - 500nm with a maximum at 470nm. The fluorescence excitation was measured in the range 200nm-500nm with the emission centred on 530nm, for Xe lamp irradiation, and 330nm for Hg lamp and laser irradiation.The fluorescence emission spectra was monitored in the spectral range 400nm – 600nm The effects of irradiation on spectroscopic characteristics of methrotrexate and fluorouracil were investigated .The irradiation was made using a UV classic lamp with Xe, for the first experimental part and for the second one it was used both a classic Hg lamp and a nytrogen pulsed laser.