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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 2:26 |
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Conference: Bucharest University Faculty of Physics 2012 Meeting
Section: Biophysics; Medical Physics
Title:
Spectroscopic studies on liposomes containing
chlorophyll A and chlorophyllide A
Authors:
S.MILENKOVIC (1), Marcela Elisabeta BARBINTA-PATRASCU (2), G.BARANGA (2), D.MARKOVIC (1), Laura TUGULEA (2)
Affiliation:
(1) Faculty of Technology, University of Nish, 16000 Leskovac, Serbia
(2) Faculty of Physics, University of Bucharest, 077125 Magurele, Romania
E-mail
milenkovic_sanja@yahoo.com; ltugulea@gmail.com
Keywords:
Chlorophyll A, Chlorphyllide A, Liposomes, Quercetin, Sspectroscopic methods
Abstract:
The aim of this work was a comparison between Chlorophyll a (Chla) and Chlorophyllide a (Chlida) incorporated in the lipid bilayers of two different types of liposomes, small unilamellar vesicles (SUV) and much bigger multilamelar vesicles (MLV), considering their spectral (visible absorption and fluorescence) characteristics. The pigments were used as sensors in liposomes, excellent biomembrane models, in order to study various effects on the lipid bilayer structures at molecular level. The VIS absorption and emission spectra of the investigated pigments were found to be different in SUV and MLV, reflecting the different localization of the cromophore in the two types of liposomes. The effect of quercetin (QCT) on liposome membrane was investigated by using both pigments, Chla and Chlida, as molecular sensors. Pursuing this goal, the fluorescence anisotropy of the two pigments was measured, while Stern-Volmer plots have been used to study the related kinetics of chromophores fluorescence quenching by quercetin. Both fluorescence polarisation data and the dynamics of the quenching process provided deeper insight regarding the QCT localization in the lipid membrane, as well as the degree of rigidity / fluidity of its environment.
Note: This work was supported under the projects number TR-34012 and OI-172044, by the Ministry of Education and Science of The Republic of Serbia and by the strategic grant POSDRU/89/1.5/S/58852, Project „Postdoctoral programme for training scientific researchers” cofinanced by the European Social Found within the Sectorial Operational Program Human Resources Development 2007–2013”
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