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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 2:00 |
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Conference: Bucharest University Faculty of Physics 2006 Meeting
Section: Optics, Spectroscopy, Plasma and Lasers
Title:
Cyanobacteria adhesion on plasma treated polyethylene terephthalate surfaces
Authors:
V. Iliosu (1), I. Ardelean (2), A. Lazea (1), L. Kravets (3), G. Dinescu (1)
Affiliation:
1()National Institute for Lasers, Plasma and Radiation Physics, PO Box MG - 16,Magurele - 077125, Bucharest, Romania;
(2) Institute of Biology of Romanian Academy, Spl. Independentei , Bucharest
3*Joint Institute for Nuclear Research, Flerov Laboratory of Nuclear Reactions, Dubna, Russia
E-mail
dinescug@infim.ro
Keywords:
polyethylene terephthalate; cyanobacteria adhesion, plasma treated polymers
Abstract:
The bacterial adhesion on polymeric surfaces is of large interest for biology and medicine, as example for obtaining infection free surfaces. The adhesion is dependent of the physical and chemical surface properties, which in turn, can be controlled by plasma processes. In this contribution results concerning cyanobacteria adhesion on plasma treated polyethylene terephthalate material are presented.
Polymeric foils (nuclear track membranes, thickness 10 m, and pore size 400 nm) were used as supports for bacteria adhesion experiments. The plasma treatments were performed in ammonia, in a parallel plate radiofrequency plasma reactor. The modification of the surface energy and surface topography were observed after ammonia plasma treatment, by contact angle measurements and Atomic Force Microscopy, respectively. This is related to the interplay between degradation of the superficial layer of the polymeric foils, re-polymerization and bonding of functional chemical groups. A biological test for adhesion and immobilization of cyanbacteria was performed. The adhesion on untreated, plasma treated surfaces was compared with the adhesion on similar surfaces but using a supplemental step of glutaraldehyde bonding as intermediate ligand factor. Optical Microscopy was used to determine the degree of adhesion. Bacteria adhesion is enhanced on the plasma treated surfaces covered/ treated with glutaraldehide. This proves for a process in which plasma treatment promotes the chemical bonding of the glutaraldehyde on the polymer surface, followed by stimulated bonding of bacteria on the glutarladehide layer.
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