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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-24 9:35 |
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Conference: Bucharest University Faculty of Physics 2011 Meeting
Section: Biophysics; Medical Physics
Title:
Extremely low frequency field effects in plant seedlings mitosis
Authors:
C. Ionita, D. Vrincianu, I. Bara, R. Focea, D. Creanga
Affiliation:
University Alexandru Ioan Cuza, Physics Department, Iasi, Romania
E-mail
cristina_elena_ionita@yahoo.com
Keywords:
cell nucleus, non-ionizing radiation, cytogenetic effects
Abstract:
In order to investigate the genotoxic effects of extremely low frequency (ELF) magnetic fields the experimental study presented inhere was focused on the identification of chromosomal aberrations in root meristemes of young seedlings of cereal species. Electromagnetic exposure was carried on freshly germinated caryopses using Helmholtz coils in laboratory arrangement with power supply from the 50 Hz network. Cytogenetic investigation was carried out based on Feulgen method using selective coloration of chromosomes with modified Carr reagent.
Microscope observation allowed the counting of all dividing cells in different mitosis phases and revealed the presence of abnormal prophases, anaphases and telophases in both exposed samples and control ones. The discussion concerning the genotoxicity of ELF magnetic field was focused on the dependence of the abnormal cell divisions on the exposure parameters. Various types of chromosomal aberrations were evidenced in the analyzed microscope slides: expelled chromosomes, interchromatidian bridges, chromosome fragments and micronuclei. Quantitative insight was obtained by means of the mitotic index calculation as well as by the assessing of abnormal percentage. Statistical signification of the changes induced by electromagnetic exposure was assured by repeated experimental measurements and statistical tests application.
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