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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 1:44 |
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Conference: Bucharest University Faculty of Physics 2013 Meeting
Section: Biophysics; Medical Physics
Title:
The irradiation/radioprotection effect in Triticum aestivum embryos
Authors:
C. Plamadeala (1), D. Vrincianu (1), I.C. Bara (2), R. Focea (3), D. Creanga (1)
Affiliation:
(1) University Alexandru Ioan Cuza, Iasi, Physics Faculty
(2) University Alexandru Ioan Cuza, Iasi, Biology Faculty
(3) University Hospital Sf. Spiridon, Iasi
E-mail
mdor@uaic.ro
Keywords:
X-ray exposure, radioprotector, plant embryos
Abstract:
The authors present an experimental study on the comparison between the cell nucleus response in irradiated vegetal embryos with and without radioprotector supply. Uniform genophond caryopses of Triticum aestivum were exposed to X-rays, with doses of 22.4 Gy, 33.6, 44.8 Gy 56.0 and 67.2 Gy. The control samples were consistent with non-irradiated caryopses in the lack of radioprotector supply (four replicates), cultivated on watered paper support while test sample series were arranged as follows: irradiated caryopses cultivated on paper support moistened with water and respectively irradiated caryopses cultivated on paper support moistened with 0.5 mL/L ethanol extract of Viola tricolor. Cytogenetic analyses were carried out on root meristemes prepared by squash technique of single cell layering and Feulgen selective dye coloration of chromosomes. Cell nucleus observation has led to both qualitative and quantitative data. From qualitative viewpoint different chromosomal abnormalities were noticed in the irradiated cells: micronuclei, laggard chromosomes, expelled chromosomes, chromosome fragments and interchromatidian bridges. Quantitatively their summative frequency was diminished up to three times for the highest radiation dose due to the radioprotector supply. The mechanism of the radioprotector action could be related to the quantitative observation of other cytogenetic index: the mitosis rate. Indeed the cell division rate was diminished for all radiation doses in the case of radioprotector supply; this empowered us to relate the radioprotector influence to the Bergonier Tribondeau law that stated that low mitosis rate is compatible to low radiosensitivity. Such radioprotector could be useful in prevention/amelioration of human irradiation representing also an available product easy to extract and administration.
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