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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 2:23 |
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Conference: Bucharest University Faculty of Physics 2015 Meeting
Section: Biophysics; Medical Physics
Title:
Proteomic analysis of plasma molecular markers as predictors of differentiated thyroid cancer
Authors:
Ancuţa E. BACIU(2,3),Viorel I. ȘUICĂ(1), Elena UYY(1), Raluca M. BOTEANU(1), Aurel POPESCU(3),
Cosmin GIULEA(4,5), Dana MANDA(2), Corin BADIU(2,5), Felicia ANTOHE(1)
Affiliation:
1) The Institute of Cellular Biology and Pathology “Nicolae Simionescu”, Bucharest, Romania
2) National Institute of Endocrinology "C. I. Parhon", Bucharest, Romania
3) University of Bucharest, Faculty of Physics, Magurele, Romania
4) Elias University Clinical Hospital, Bucharest, Romania
5) “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
E-mail
ancutaelenabaciu@yahoo.com
Keywords:
Keywords: differentiated thyroid cancer, microvesicles, plasma proteins, mass spectrometry, molecular markers.
Abstract:
Objective
Differentiated thyroid cancer (DTC) derived from follicular epithelium is the most frequent endocrine cancer, that presents a different biological behaviour and an aggressive cell proliferation. The aim of this study was to identify a panel of potential biomarkers for early detection, diagnosis and prognosis of differentiated thyroid cancer.
Materials and methods
The proteins were isolated from EDTA blood samples, prior to surgery, from 40 patients with thyroid neoplasia, after their informed consent. The studied groups were the following: differentiated thyroid carcinoma (DTC), follicular adenoma (A), and controls (C) each of 10 individuals. The plasma purified microvesicle samples were separated by nano-liquid chromatography and analyzed by mass spectrometry. Tumour and non-tumour adjacent tissue samples were harvested as well. Protein identification, quantification and characterization were performed using dedicated bioinformatics systems.
Results and discussions
The data analysis allowed the identification in microvesicle samples of 1,218 proteins classified by Gene Ontology as extra-cellular (~20 %), membrane (~ 15 %), cytoskeletal (~ 10 %), and nuclear (~12 %) proteins involved mainly in the metabolic processes, regulation of the biological processes, and catalytic activity; 215 of them were uniquely identified in DTC group and 253 in A group. It is interestingly to note that 198 proteins were present in both DTC and A groups. The potential evolution of the DTC was predicted by correlations between tissue and plasma proteomic analysis.
Conclusions
The presence of thyroid neoplasia induced changes in tissue and plasma protein profiles of microvesicles, documented by HPLC and tandem mass spectrometry.
Acknowledgement:
The present work was supported by the Romanian Academy PN II CNDI – UEFISCDI, Project no.:135/2012. A. E. Baciu was supported by the project: POSDRU/159/1.5/S/137750,”Project Doctoral and Postdoctoral programs support for increased competitiveness in Exact Sciences Research” co-financed by the European Social Found within the Sectoral Operational Program Human Resources Development 2007 – 2013.
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