Faculty of Physics - University of Bucharest

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FACULTY OF PHYSICS

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2024-11-24 23:06

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Conference: Bucharest University Faculty of Physics 2015 Meeting

Section: Polymer Physics

Title:

Degradable thin films containing Lactoferrin and Cysplatin effect on murine melanoma cells

Authors:

Valentina DINCA (1),Valentina MARASCU (1, 3), Madalina ICRIVERZI (2), Laurentiu RUSEN (1), Anca ROSEANU (2), Maria DINESCU (1)

Affiliation:

1) INFLPR, National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Boulevard, PO Box MG-16, RO-077125 Magurele, Bucharest, Romania

2) IBAR, Institute of Biochemistry of the Romanian Academy, 296 Splaiul Independentei, RO-060031 Bucharest, Romania

3) Faculty of Physics, University of Bucharest, 405 Atomistilor Street, 077125 Bucharest- Magurele, Romania

E-mail

valentina.dinca@inflpr.ro

Keywords:

Lactoferrin, FTIR, melanoma cells, MAPLE

Abstract:

Functional Lactoferin and Cysplatin were prepared through the matrix assisted pulsed laser evaporation (MAPLE) technique. The MAPLE technique involves focusing a pulsed laser beam (266 nm, fluence. 0.2 to 0.7 J/cm2) onto a frozen target consisting of the organic molecules dissolved/dispersed in a solvent matrix. The experiments take place in vacuum. The laser beam evaporates the surface layers of the target, with both solvent and solute molecules being released into the chamber. The volatile solvent is pumped away, whereas the organic molecules coat the substrate. Thin uniform films of Lf, Cys and Lf mixed with Cys, have been deposited on Si and Permanox substrates. The films prepared using this method have been examined by optical microscopy, scanning electron microscopy, atomic force microscopy and Fourier transform infrared spectroscopy. Careful control of the processing conditions allowed the complex polymer/organic molecules to be transferred to the substrate as uniform without any significant chemical modification. The obtained results demonstrated the full functionality of the Lf and Cys thin films obtained by MAPLE, which allowed us to prove the viability of MAPLE for the production of proteins, mixtures of protein and citostatics compounds thin films for obtaining active antitumoral coatings.