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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 1:40 |
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Conference: Bucharest University Faculty of Physics 2015 Meeting
Section: Biophysics; Medical Physics
Title:
Gold nanoparticles functionalized with 68Ga-DOTA-conjugated peptides for molecular imaging
Authors:
Elena L. CHILUG(1,2), Marcela E. BARBINTA-PATRASCU(1), Alexandru C. ION(1,3), Radu LEONTE(2,4), Alina RAICU(2), Catalin TUTA(2) and Dana NICULAE(2)
Affiliation:
(1) University of Bucharest, Faculty of Physics, 405 Atomistilor Street, P.O. Box MG-11, Bucharest-Magurele, 077125, Romania
(2) National Institute of Physics and Nuclear Engineering Horia Hulubei, P.O.BOX MG-6, Bucharest – Magurele, Romania
(3) Research and development center for nanotechnology AQUANANO (AQUANANO is a registered trademark of AGHORAS INVENT SRL), Romania
(4) University Politehnica of Bucharest, Romania
E-mail
elipatras@gmail.com
Keywords:
Gold nanoparticles, radiolabeling, peptides, cancer, PET
Abstract:
Radiolabelled peptides constitute a promising tool for cancer diagnosis based on their specific tumour targeting characteristics. Binding radiopharmaceuticals on the surface of various metal nanoparticles could improve imaging of tumour over-expressing specific receptors. The aim of this research was to evaluate in vitro the uptake and retention of gold nanoparticles (AuNPs) functionalized with 68Ga-radiolabeled peptides, by colon and pancreatic tumour cell lines HT-29 and AR42J. Different techniques (DLS and zeta potential measurements, absorption spectroscopy) were used to characterize AuNPs prior to the biological assessment. The formation of gold-based radiolabelled bioconjugates was checked by UV-Vis absorption spectroscopy. This study revealed an improved retention of Ga-68 radioisotope in the presence of AuNPs.
Acknowledgement:
The work has been funded by the Sectorial Operational Programme Human Resources Development 2007-2013 of the Ministry of European Funds through the Financial Agreement POSDRU/159/1.5/S/134398, and by PCCA 2014 contract 228.
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