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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 1:24 |
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Conference: Bucharest University Faculty of Physics 2015 Meeting
Section: Polymer Physics
Title:
FTIR and TEM study regarding the effect of Coca-Cola regular beverage on the human dental enamel: an in vitro study
Authors:
Victor CIUPINA (1,3), Valentina MARASCU (2,4), Agripina ZAHARIA (1), Gabriel PRODAN (1), Cristian LUNGU (2), Corneliu POROSNICU (2), Aureliana CARAIANE (1), Valentin BARNA (4), Gheorghe DINESCU (2,4)
Affiliation:
1) Ovidius University of Constanta, 124 Mamaia Avenue, Constanta, Constanta, Romania 900527
2) National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Bucharest– Magurele, Romania
3) Academy of Romanian Scientists, Splaiul Independentei No. 54, Bucharest 050094, Romania
4) Faculty of Physics, University of Bucharest, 405 Atomistilor, 077125 Bucharest- Magurele, Romania
E-mail
vciupina@yahoo.com, gprodan@univ-ovidius.ro, valentina.marascu@gmail.com
Keywords:
Enamel dental specimens, Coca-Cola, Hydroxyapatite, FTIR, TEM
Abstract:
In our work we study the effect of Coca-Cola on the human dental enamel by using Jasco FTIR 6300 spectrometer with MIRacleTM Single Reflection Horizontal ATR Accessory, designed for single reflection attenuated total reflectance ATR and TEM techniques, using a CM120ST microscope. Human dental enamel is composed by hydroxylapatite, also called hydroxyapatite (HA), which is also present in the human bones. Hydroxyapatite is a naturally occurring mineral form of calcium, phosphorous, oxygen and hydrogen atoms. Seven healthy teeth were used to prepare seven samples of human enamel. Six teeth were immersed in Coca-Cola for 5, 15, 30, 60, 120 and 180 minutes respectively. All samples were placed on the ATR crystal diamond in a fix position and were measured at 4cm-1 resolution. For TEM measurements, the teeth were grind by means of diamond drill at 5000-10000rpm. Powder was then milled in agate mortar and finally dispersed in alcohol and put on the formvar covered Cu grid. Diffraction pattern (SAED) images were performed in areas with maxima information. The crystallite dimension was estimated using Debye-Scherrer formula. FTIR spectra results proves that, due to regular beverage, caffeine, from Coca-Cola, has a strong influence on the human enamel. For higher time exposures, the teeth gain a specific colour from the liquid. FTIR spectra reveals that a number of components of liquid (Coca-Cola) remain unchanged within the enamel; there are a number of components (C, O) that overlap or are attached to existing configurations in the structure of tooth enamel. Such a statement is consistent with the results of TEM that signals a change in the dimensions of crystallites of hydroxyapatite because of immersion in Coca-Cola.
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