UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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Conference: Bucharest University Faculty of Physics 2015 Meeting


Section: Solid State Physics and Materials Science


Title:
Study of the stability of BSA/PEDOT:PSS mixture solution as part of biosensing devices platform


Authors:
O. BRĪNCOVEANU(1,2), A. IOANID(1), S. IFTIMIE(1), M. ENACHESCU(2), S. ANTOHE(1)


Affiliation:
1) Faculty of Physics, University of Bucharest, 077125, Romania

2) Center for Surface Science and Nanotechnology, University Politehnica of Bucharest, 060042 Romania



E-mail
oana_brincoveanu@yahoo.com


Keywords:
biosensors, BSA, PEDOT-PSS, stability


Abstract:
Bovine serum albumin : poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (BSA:PEDOT-PSS) solutions with the molar ratio 10:1 were prepared and deposited as a bioactive layer. This bioactive layer is part of the structure of a biosensor with the abilities to detect, to identify, and to immobilize biomolecular structures present in physiological fluids like blood, interstitial fluid, cell culture media. Bioactive surface adhesion depends on the specific nature of the interactions between biomolecule and the active layer. The sensitivity and efficiency of the process is influenced by temperature, time and the nature of the environment. UV-Spectroscopy analysis of the mixture solutions was performed for various concentration ratios and their evolution in time (clinical), at room temperature. Moreover, thermal analysis (DSC) and thermogravimetric (TGA) characterization showed good stability in air and nitrogen atmosphere.


References:

A novel electrochemical biosensing platform based on poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) composites , Yangping Wen, Jingkun Xu, Dong Li, Ming Liu, Fangfang Kong, Haohua He, Synthetic materials 162 , p 1308-1314, 2012

Biomaterials: Protein-Surface Intweractions, Robert A. Latour, Jr., Enciclopedia of Biomaterials and Biomedical Engineering, 2005.



Acknowledgement:
The work has been funded by the Sectoral Operational Programme Human Resources Development 2007-2013 of the Ministry of European Funds through the Financial Agreement POSDRU/159/1.5/S/137750.