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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-23 18:06 |
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Conference: Bucharest University Faculty of Physics 2016 Meeting
Section: Biophysics; Medical Physics
Title:
Biogenic Ferrihydrite Nanoparticles Produced by Bacteria Klebsiella oxytoca in Interaction with Serum Albumins
Authors:
Claudia G. CHILOM(1), Virginia DINCA (1,2), Doina M. GAZDARU (1),
Aurel I. POPESCU(1), Maria BALASOIU (3)
Affiliation:
1) Department of Electricity, Solid Physics and Biophysics, Faculty of Physics, University of Bucharest, Romania
2) Ovidius University, Constanşa, Romania
3) Joint Institute for Nuclear Research Dubna, Russia
E-mail
claudiafir@gmail.com
Keywords:
Bacterial nanoparticles, albumins-ferrihydrite nanoparticles interaction, spectroscopic methods.
Abstract:
FThe ferrihydrite nanoparticles are biogenic compounds produced by Klebsiella oxytoca, a gram-negative bacterium, by biomineralization of iron salt solutions. These nanoparticles present interesting magnetic properties that seems to be useful in nanomedicine and bioengineering.
Some spectroscopic properties of ferrihydrite nanoparticles and their interaction with human and bovine serum albumins were investigated by UV-Vis spectroscopy and steady-state fluorescence spectroscopy.
The absorption spectra of diluted samples of biogenic ferrihydrite nanoparticles dispersed in HEPES buffer show a large optical s density in the 250 - 400 nm spectral range.
UV-Vis spectra show also a small spectral shift of the absorption maximum and a change in the optical density of the tryptophan residues from proteins, in the presence of ferrihydrite nanoparticles. This is an indication that the ferrihydrite nanoparticles bind the protein sites with weak or moderate strength.
The fluorescence emission spectra of the complexes show that albumin-ferrihydrite nanoparticle interaction is moderate. Thermal stability of the albumin structure to the denaturation, monitored by fluorescence emission decay, is very little improved in the presence of ferrihydrite nanoparticles.
Acknowledgement:
Acknowledgement: The work was accomplished with the financial support of the IUNC “Structural and spectrophotometric characterization of biogenic systems”, Grants 4-1069-2009/2014 and 04-4-1121-2015/2017.
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