UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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2024-11-23 18:06
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Conference: Bucharest University Faculty of Physics 2016 Meeting


Section: Biophysics; Medical Physics


Title:
Investigating Anticancer Activity of Biogenic Ferrihydrite Nanoparticles Produced by Bacteria Klebsiella oxytoca


Authors:
Claudia G. CHILOM (1), Mihaela BACALUM (2), Doina M. GAZDARU (1), Aurel I. POPESCU (1), Maria BALASOIU (3)


Affiliation:
1) Department of Electricity, Solid Physics and Biophysics, Faculty of Physics, University of Bucharest, Romania

2) Institutul Naţional de Cercetare-Dezvoltare pentru Fizică ṣi Inginerie Nucleară „Horia Hulubei (IFIN-HH), Romania

3) Joint Institute for Nuclear Research Dubna, Russia


E-mail
claudiafir@gmail.com


Keywords:
Bacterial nanoparticles, cell lines, cytotoxicity, hemolytic effect


Abstract:
The ferrihydrite nanoparticles are biogenic compounds produced by biomineralization of iron salt solutions by Klebsiella oxytoca, a gram-negative bacterium. Analysis of these nanoparticles showed their interesting magnetic properties that could be useful in nanomedicine and bioengineering. The aim of this study is to evaluate the toxic effects against eukaryotic cells of the ferrihydrite nanoparticles. In order to study the cytotoxicity of the biogenic nanoparticles against four different cell lines, the MTTassay (a colorimetric assay for assessing cell metabolic activity) was used. The cell lines used are: one healthy cell line - L929 (mouse fibroblasts) and three tumour cell lines, namely, HT-29 (human colorectal adenocarcinoma cell), HepG2 (human liver carcinoma cells) and MG-63 (human osteosarcoma).We also evaluated nanoparticles’ability to cause hemolysis. The nanoparticles show a lower toxicity against the L929 cells, as compared to the three tumour cells lines. From the three tumour lines, the ferrihydrite nanoparticles proved to be more toxic against MG-63 cells. No hemolytic effects were observed for the tested concentrations.


Acknowledgement:
Acknowledgement: The work was accomplished with the financial support of the IUNC “Structural and spectrophotometric characterization of biogenic systems”, Grants 4-1069-2009/2014 and 04-4-1121-2015/2017.