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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-23 17:40 |
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Conference: Bucharest University Faculty of Physics 2016 Meeting
Section: Biophysics; Medical Physics
Title:
An Approach for Low Dose Radiotherapy Improvement Using Functionalized Magnetite Nanoparticles
Authors:
R. C. POPESCU (1,2), E. ANDRONESCU (2), A. I. APOSTOL (3,4), M. STRATICIUC (5), G. VOICU (2), L. MOGOANTA (6),
G. D. MOGOSANU (7), R. TRUSCA (2), B. VASILE (2),
A. M. GRUMEZESCU (2), I. PETCU (1), D. SAVU (1)
Affiliation:
(1) “Horia Hulubei” National Insitute of Physics and Nuclear Engineering, Department of Life and Environmental Physics
(2)“Politehnica” University of Bucharest, Department of Science and Engineering of Oxide Materials and Nanomaterials
(3)“Horia Hulubei” National Insitute of Physics and Nuclear Engineering, Department of Nuclear Physics
(4) Faculty of Physics, University of Bucharest, Magurele Romania
(5) “Horia Hulubei” National Insitute of Physics and Nuclear Engineering, Department of Applied Nuclear Physics
(6) University of Medicine and Pharmacy of Craiova, Research Center for Microscopic Morphology and Immunology
(7) University of Medicine and Pharmacy of Craiova, Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy
E-mail
roxana.popescu@nipne.ro
Keywords:
Low dose radiotherapy, magnetite nanoparticles, magnetic targeting, PIXE entrapment efficiency
Abstract:
The tumour resistance to chemotherapy and/or radiotherapy was attempted to be exceeded by combining these two methods of treatment. However, the results were not as expected. Nowadays, nanotechnology presents high interest in many biomedical applications, one of them being the fabrication of targeted carriers for anti-tumour substances in cancer therapy. Here, we propose a new approach for cancer treatment, which involves low dose radiotherapy to increase the cancer cells sensibility and permeability for biocompatible magnetite nanoparticles. These nanoparticles are targeted at the tumour site by magnetic guiding, where they carry very low doses of doxorubicin (DOX), a chemotherapeutic substance.
The doxorubicin-conjugated magnetite nanoparticles (Fe3O4@DOX) were identified by XRD (X-ray diffraction) and SAED (selected area diffraction), crystallinity being also proved through HRTEM (high resolution transmission electron microscopy. The TGA (thermogravimetric analysis) and FTIR (Fourier transform infrared spectroscopy) emphasized the interaction between the nanoparticles and DOX.
For the biological evaluation, MG-63 human osteosarcoma cells were used as in vitro model, the system mechanisms of action being evaluated (cytotoxicity assays, immunofluorescence microscopy, and scanning electron microscopy, genotoxicity evaluation and particle-induced X-ray emission for nanoparticles entrapment efficiency in cells). The in vivo biodistribution proved the biocompatibility of these systems with living tissues for BALB-c mice models and chick embryos models. The active magnetic targeting capability was in vitro proved, while 1 Gy ionizing radiation was applied prior to the nanoparticles treatment for in vitro models, resulting in improved toxic effects against cancer cells.
Acknowledgement:
This study was supported by the Romanian Ministry of Education and Research [National Grants No. PN 09370301, PN-II-ID-PCCE-2011-2-0027, PN-II-123/2012 and PN-II-PCCA-109/2014].
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