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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-23 17:38 |
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Conference: Bucharest University Faculty of Physics 2016 Meeting
Section: Optics, Spectroscopy, Plasma and Lasers
Title:
Detection of epidermoid squamous-cell carcinoma by laser induced autofluorescence
Authors:
R. COSTIN (1), Tatiana TOZAR(2), V. ZAINEA(3), Ruxandra PIRVULESCU(4), I. R. ANDREI(2)
Affiliation:
1) Central Military University Emergency Hospital Dr. Carol Davila, Bucharest
2) National Institute for Laser, Plasma and Radiation Physics, Magurele
3) Institute of Phonoaudiolology and ENT Functional Surgery “Prof.Dr. D. Hociota”, Bucharest
4) Ophthalmology Clinic, University of Medicine and Pharmacy “Carol Davila”, Bucharest
E-mail
cromeorl@yahoo.com,ionut.andrei@inflpr.ro
Keywords:
LIF, epidermoid squamous-cell carcinoma, autoinduced fluorescence
Abstract:
Epidermoid squamous-cell carcinoma is the most common malignant tumor of larynx (95-96%), representing 1.6-2% of cancers in men and 0.2-0.4% of malignancies in females, with a world growing incidence. By exposing cells and tissues to UV light, the excitation of naturally occurring chromophores occurs in part by non-radiative deactivations, in part via fluorescence emission. Using laser induced fluorescence (LIF) in natural tissues not impregnated with photosensitizers as a noninvasive autofluorescence technique for both diagnosis and intraoperative assessments of laryngeal cancer we can improve the tumor malign identification in vivo.
A total of three laryngeal biopsies (i.e. three pairs of tissues, each pair containing a healthy and a tumor tissue sample extracted from the same patient) were considered in this study. The samples were collected from patients previously diagnosed with stage III-IV laryngeal carcinoma. Immediately after the surgery fragments of normal tissue and neoplastic tissue were collected, fragments of which later, after freezers, were sectioned in 20-30μm thickness slices and stretched to quartz slides. All samples were subjected to controlled laser irradiation using a pulsed diode laser (ʎ=375nm, pulse width=87ps, frequency 31MHz) and the fluorescence and its lifetime were collected using two optical fibers (inner diameter 400µm and 1500µm, respectively) positioned in a 45o geometry. The signals were recorded using a spectrograph and a photo-sensor module, the output of which was fed to a digital oscilloscope.
We assessed the impact of laser induced fluorescence and fluorescence lifetime measurements in order to identify the differences between healthy and tumoral laryngeal tissue and outlining them, in terms of differences between the laser autoinduced fluorescence averaged intensity. The results determined the usefulness of laser induced spectroscopy in the diagnosis of laryngeal squamous cell carcinoma, discriminating between the malignant and normal tissue the by analyzing the differences in spectral fluorescence intensity and fluorescence lifetime.
Acknowledgement:
This work has been financed by the National Authority for Research and Innovation in the frame of Nucleus programme - contract 4N/2016 and the project number PN-II-ID-PCE-2011-3-0922.
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