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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-23 18:15 |
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Conference: Bucharest University Faculty of Physics 2017 Meeting
Section: Optics, Spectroscopy, Plasma and Lasers
Title:
Uv-Vis and FTIR spectroscopic investigations of gamma - ray irradiated antibiotics
Authors:
Adriana SMARANDACHE(1), Ralf MOELLER(2), Mihail Lucian PASCU(1,3)
Affiliation:
1) National Institute for Laser Plasma and Radiation Physics, Str. Atomistilor 409, 077125 Magurele, Romania;
2) German Aerospace Center (DLR e.V.), Linder Hoehe , 51147 Cologne (Köln), Germany;
3) Faculty of Physics, University of Bucharest, Str. Atomistilor 405, 077125 Magurele, Romania.
E-mail
adriana.smarandache@inflpr.ro
Keywords:
UV-VIS absorption spectra, FTIR spectroscopy, gamma irradiation, Rifampicin, Nalidixic acid, Spectinomycin
Abstract:
In modern medicine, a number of sterilization methods are applied, including gamma – ray irrradiation. Radiation sterilization is especially useful in the case of thermolabile products, because irradiation causes only a small rise in the temperature of sterilized substances [1]. In the case of gamma radiation, a substance to be sterilized does not directly interact with the reagents and, as a result, lacks any traces of chemical pollution [2]. The aim of this work was to determine the influence of gamma irradiation on three antibiotics (Rifampicin, Nalidixic acid, and Spectinomycin) using UV-VIS and FTIR spectroscopy methods. Different radiation doses (up to 204 kGy) from encapsulated 60Co source have been applied to the crystalline solid samples of antibiotics. The control samples have been either stored in laboratory (at room temperature and protected from environment light), at the irradiation facility in the same conditions and in the fridge (4-8°C and protected from environment light). The spectral properties of both control and gamma irradiated antibiotics were measured using an UV-VIS-NIR spectrophotometer (Lambda 950, Perkin Elmer, USA) and a FTIR spectrophotometer (Nicolet IS50, Thermo Scientific, USA). The extent of the radio-induced degradation was limited for Rifampicin and Nalidixic acid, according to their UV-VIS spectra evolution. These drugs might be good candidates for radiosterilization, unlike the Spectinomycin, for which the strongest molecular modifications were noticed either for control kept in dark at room temperature or for gamma irradiated samples. Vibrational changes occurred for the three antibiotics on which irradiation doses of 6 kGy and 24 kGy have been applied. Also, many of these modifications are registered for the control samples kept in dark, at room temperature at irradiation facility. In a molecular irradiated solid, the radiosensitivity is expressed by the formation of radicals, so one of the possible next steps consists on side-products identification.
References:
1. Basly JP, Basly I, Bernard M (1998b) Electron spin resonance identification of irradiated ascorbic acid: dosimetry and influence of powder fineness. Anal Chim Acta 372:373–378.
2. Katusin-Razem B, Hamitouche K, Maltar-Strmecki N (2005) Radiation sterilization of ketoprofen. Radiat Phys Chem 73:111–116.
Acknowledgement:
Aknowledgements: The authors acknowledge the financial support of the ANCS by project number PN-III-P2-2.1-PED-2016-0420, and the PN Nucleu project: 1647/2016.
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