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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-23 18:11 |
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Conference: Bucharest University Faculty of Physics 2017 Meeting
Section: Polymer Physics
Title:
Multifunctional copolymeric - ceramic composite coatings obtained by Matrix Asssisted Pulsed Laser Evaporation
Authors:
Valentina DINCA(1), Laurentiu RUSEN(1), Simona BRAJNICOV(1,3), Patricia NEACSU(4), Valentina MARASCU(1,2), Anca BONCIU(1,2), Anisoara CIMPEAN(3), Maria DINESCU(1)
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Affiliation:
1)National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, PO Box MG-16, 077125, Magurele, Bucharest, Romania
2)University of Bucharest, Faculty of Physics, 405 Atomistilor, 077125 Bucharest- Magurele, Romania
3)University of Craiova, Faculty of Mathematics and Natural Science, 13 Alexandru Ioan Cuza, 200585 Craiova, Romania
4)University of Bucharest, Department of Biochemistry and Molecular Biology, 91-95 Spl. Independentei, 050095, Bucharest, Romania
E-mail
valentina.marascu@gmail.com
Keywords:
copolymer, ceramic composite, pulsed laser evaporation
Abstract:
An essential requirement for a candidate material to significantly improve the speed of bone formation on its surface is the ability to promote bone matrix maturation and mineralization. Nevertheless, the surface characteristics of materials surface in terms of topography, surface energy or chemistry, play an important role in cell behavior having implications over long term cell-substrate interactions both in vitro and in vivo1. The objective of our study was to evaluate the in vitro response of MC3T3-E1 pre-osteoblasts to multifunctional poly(ethylene glycol)-block-poly(ε-caprolactone) methyl ether-hydroxyapatite (PEG-PCL-HA) coatings deposited by Matrix Assisted Pulsed Laser Evaporation technique. These chemical and morphological characteristics of the coatings were analyzed by FTIR, AFM and SEM analysis and correlated to materials composition and cellular response. The cell culture-based studies have been performed in terms of cell viability/proliferation, cell adhesion and morphology and osteoblast differentiation (extracellular matrix mineralization, alkaline phosphatase activity and expression levels of collagen). Lactate dehydrogenase activity, assessed in cell culture media as a relevant toxicity marker, recorded an almost constant low level over 1-, 3- and 5-day culture periods for all analyzed coatings, suggesting that they are biocompatible. Moreover, these coatings promoted cell proliferation in similar extent as established by MTT assay. Fluorescent staining of actin cytoskeleton and vinculin at 4h post-seeding demonstrated that MC3T3-E1 cells equally spread on all coatings excepting HA coating where they were less spread. However, at 24h post-seeding the cells adopted typical osteoblast morphologies with well-defined stress fibers and almost similar cell density. The extracellular matrix mineralization was quantified by Alizarin red staining at 2 and 4 weeks after cell seeding both in standard and pro-osteogenic (stimulation with ascorbic acid and β-glycerophosphate) culture conditions. Our in vitro findings indicate that the multifunctional PEG-PCL-HA coatings elicit a positive osteoblast response showing promise for future biomedical applications.
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