UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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2024-11-23 18:18
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Conference: Bucharest University Faculty of Physics 2017 Meeting


Section: Biophysics; Medical Physics


Title:
Biocompatibility study of magnetite nanoparticles synthesized by a green method


Authors:
Mihaela TEMELIE (1), Roxana Cristina POPESCU (1,2), Diana COCIOABA (1,3), Bogdan Stefan VASILE (2), Diana SAVU (1)


*
Affiliation:
1. National Institute of Physiscs and Nuclear Engineering "Horia Hulubei", Magurele, Romania

2. Polytechnic University of Bucharest, Bucharest, Romania

3. University of Bucharest, Bucharest, Romania


E-mail
roxana.popescu@nipne.ro, dsavu@nipne.ro


Keywords:
Magnetite nanopaticles, turmeric, L929, biocompatibility


Abstract:
Turmeric (Curcuma longa) was used many years in food service but also as a therapeutic agent for a wide range of diseases. Curcumin, the active compound of turmeric, was widely studied being proved to have antibacterial, anti-inflammatory, antitumoral, antioxidant effects and many other beneficial properties. The aim of our study was to characterize and perform a biocompatibility evaluation for a new synthesized magnetite nanoparticles, obtained by a less pollutant turmeric-assisted method. Physical-chemical characterization was done using transmission electron microscopy and selected area electron diffraction in order to prove the high crystalinity of the nanoparticles and to identify the component phase. The in vitro biocopatibility studies were performes on normal fibroblasts (L929 cell line). Trypan blue and morphological apoptosis observation proved that the new magnetite nanoparticles do not induce cellular death. Micronucleus scoring indicates no genotoxic effect. The MTT test showed a transient decrease in metabolic capacity at 24 hours of incubation with the new synthetized magnetite nanoparticles, but the cells regain quickly the normal function. The decrease in metabolic capacity was correlated with a temporary block of cell cycle. It might be speculated that the cells need a time interval to process the material. Hemolysis assay showed no erythrocyte lysis. Our experiments conclude that the new syntesized nanoparticles obtained by a green method represent a biocompatibile material.