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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 2:12 |
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Conference: Bucharest University Faculty of Physics 2018 Meeting
Section: Polymer Physics
Title:
Biodegradable interfacing nanocomposite coatings for modulating the cellular response
Authors:
Anca BONCIU (1,2), Simona BRAJNICOV (1,3), Luminita Nicoleta DUMITRESCU (1,3), Valentina MARASCU (1,2), Laurentiu RUSEN (1), Valentina DINCA (1), Maria DINESCU (1), Anisoara CIMPEAN (4)
Affiliation:
1) National Institute for Lasers, Plasma and radiation Physics, Bucharest, Romania
2) University of Bucharest, Faculty of Physics, Romania
3) University of Craiova, Faculty of Mathematics and Natural Sciences, Craiova, Romania
4) University of Bucharest, Faculty of Biology, Bucharest, Romania
E-mail
valentina.dinca@inflpr.ro
Keywords:
biodegradable materials, ceramic nanoparticles, nanocomposite coatings, MAPLE, AFM, SEM, FTIR
Abstract:
Various design and functionalization strategies are used nowadays for obtaining multifunctional coatings based on biodegradable and biocompatible materials for targeting cells activity and enhancing the bio-response. Active compounds including from carbon based materials, ceramics to proteins are used for enhancing cellular response. In the last years, recent studies showed that the distribution of ceramic nanoparticles and other bioactive compounds presence have significant influence for nano-composites interfaces for osteoblast response envisaging osseous implant application. Therefore, this work is focused on embedding HA spherical nanoparticles and lactoferrin (LF) within synthetic biodegradable copolymers Poly(ethylene glycol)-block-poly(ε-caprolactone) methyl ether (PEG-block-PCL Me) for the preparation of new nanocomposites coatings targeting the modulated response of osteoblast cells (i.e adhesion, mineralization). The controlled incorporation of HA and LF within the synthetic copolymeric substrates was performed by matrix assisted pulsed laser evaporation (MAPLE) method using a modular target system. The resulting morphologies and the main features were studied by Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM). Fourier Transform Infrared Spectroscopy (FTIR) data demonstrates that the functional groups in the MAPLE-deposited films remain intact for the individual compounds and that LF was not affected by the solvents used for copolymer. The behaviour of the coatings during immersion experiments were evaluated and correlated to the bio-functionality of the coatings. The results clearly revealed that the coatings with HA and LF incorporated in the polymeric matrix have enhanced stability as compared with single element coatings, and that the MC3T3-E1 murine osteoblasts response in vitro (cell adhesion/morphology, proliferation and matrix mineralization) was differentially influenced by the variations in the physicochemical characteristics of materials biointerface.
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