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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 1:53 |
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Conference: Bucharest University Faculty of Physics 2018 Meeting
Section: Biophysics; Medical Physics
Title:
The interaction of nanometric tungsten dust with fibroblasts cells
Authors:
Lavinia Gabriela CARPEN (1,2), Maria Adriana ACASANDREI (3), Diana Iulia SAVU (3), Tomy ACSENTE (1), Elena MATEI (4), Claudia Gabriela CHILOM (2), Gheorghe DINESCU (1,2)
Affiliation:
1) INFLPR, Atomiștilor 409 Street, Bucharest- Măgurele, Romania
2) University of Bucharest, Faculty of Physics, Atomiştilor 405 Street,
Măgurele, Bucharest, 077125, Romania 3) IFIN-HH, Reactorului Street, No.30, P.O. BOX MG-6, Bucharest - Măgurele, ROMANIA 4) NIMP, Atomiștilor Street, No. 405A P.O. Box MG 7, 077125, Măgurele, Romania
lavinia.carpen@infim.ro
E-mail
lavinia.carpen@infim.ro
Keywords:
tungsten nanoparticles, cytotoxicity, fibroblast cells
Abstract:
In future fusion devices, plasma interaction with Tokamak tungsten walls can trigger the dust formation leading to different type of issues, including plasma perturbation and safety ones. According to the scenario for a loss of vacuum accident (LOVA) [1], in the case of confinement failure, the dust can be released in nuclear plant vicinity. Thus, these tungsten particles can be harmful for fusion workers. To evaluate their possible toxicity, tungsten nanoparticles were produced as laboratory model for Tokamak like dust, using magnetron sputtering gas aggregation technique [2]. To investigate the tungsten dust cytotoxicity, particles in different concentrations were used. According to the purpose of this research, the epithelial human cells (BJ ATCC) were chosen to evaluate the nanoparticle toxic effect. The cytotoxicity effect was observed using a MTS (3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) viability assay on cells. The metal nanoparticle interaction with fibroblasts was evaluated using the scanning electron microscopy. By using secondary electron imaging in comparison with high-sensitive backscattered electron imaging, a detailed characterization of processes of nanoparticle internalization into cells was obtained. It was demonstrated that, at high concentration (upper than 100 µg/mL), the tungsten nanoparticles present toxic effects on fibroblast cells.
References:
[1] A. Malizia et al., Energies (2016) 9, 578
[2] T. Acsente et al., Eur. Phys. J. D 69 (2015) 161
Acknowledgement:
We acknowledge the financial support in the frame of the Projects: NUCLEU-INFLPR - 2018, PN-16420203, Projects EUROfusion Consortium WPEDU-RO, WPENR STANDS and IFA-CEA C5-07.
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