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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 1:36 |
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Conference: Bucharest University Faculty of Physics 2018 Meeting
Section: Physics and Technology of Renewable and Alternative Energy Sources
Title:
The investigation of yeast cells using Atomic Force Microscopy
Authors:
Alexandra SERBAN (1), Adriana BALAN (2)
Affiliation:
1) University of Bucharest, Faculty of Physics, P.O.Box MG-11, Magurele, 077125 Bucharest, Romania
2) 3Nano-SAE Research Center, PO Box MG-38, Bucharest-Magurele, Romania
E-mail
alexandra_serban1997@yahoo.com
Keywords:
atomic force microscopy, adhesion forces, nanomechanical properties, Saccharomyces cerevisiae
Abstract:
Saccharomyces cerevisiae are widespread in the scientific community for versatile applications, the main reason being that they have complex internal cell structure. In addition, yeast cells are easy to manipulate, they are widely available and rapidly reproduce.
In this study, nanomechanical properties of fungi Saccharomyces cerevisiae were investigated with the Atomic Force Microscopy (AFM). Bacteria were deposited on freshly cleaved mica and studied in adhesion of AFM tip to the surfaces of Saccharomyces cerevisiae. The method proposed, i.e. direct deposition of microorganisms onto the freshly cleaved mica substrate, has the advantage of being easy to use without any sample or substrate pretreatment.
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