UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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2024-11-22 1:57
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Conference: Bucharest University Faculty of Physics 2021 Meeting


Section: Optics, Spectroscopy, Plasma and Lasers


Title:
Ocular tumor tissues imaging with white light diffraction phase microscopy


Authors:
Viorel NASTASA (1,†), Adriana SMARANDACHE (2,*,†), Ruxandra A. PIRVULESCU (3,4, †), Ionut-Relu ANDREI (2), George SIMION (3,4), Gabriel POPESCU (5), Mihail-Lucian PASCU (2,6)


Affiliation:
1) Extreme Light Infrastructure - Nuclear Physics ELI-NP, “Horia Hulubei” National Institute for Physics and Nuclear Engineering IFIN-HH, 077125 Magurele, Romania;

2) National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania;

3) University of Medicine and Pharmacy “Carol Davila”, 020022 Bucharest, Romania;

4) Emergency University Hospital Bucharest, 050098 Bucharest, Romania

5) Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, 61801, Illinois, USA;

6) Faculty of Physics, University of Bucharest, 077125, Bucharest, Romania


E-mail
adriana.smarandache@inflpr.ro


Keywords:
eye tumor; white light diffraction phase microscopy; quantitative phase imaging; phase map; refractive index


Abstract:
In the current context of histopathology, where the gold standard relies on manual investigation of stained tissues [1], the development of new sensitive and quantitative methods for early disease diagnosis and automatic screening of samples is required. It has been found in the last decades that by combining holography and microscopy, highly sensitive measurements, capable of detecting very small refractive index variations of thin biological specimens, can be achieved [2], [3]. This paper aims to perform a comparative analysis of the limits of surgical resection of eye tumors by both conventional and white light diffraction phase microscopy, a quantitative phase imaging technique. Sample images were acquired by microscopy in bright field and in phase contrast, coupled with white light diffraction phase microscopy. A comparative histological analysis was performed to evidence the characteristics of biological structures. We showed that white light diffraction phase microscopy can reveal cellular and subcellular structures in transparent tissue slices based on the refractive index distribution. This study responds to the current need for providing quantitative data from the obtained images at larger penetration depth with good contrast, down to molecular specificity.


References:

[1] “Milestones in light microscopy,” Nat. Cell Biol., vol. 11, no. 10, pp. 1165–1165, Oct. 2009, doi: 10.1038/ncb1009-1165.

[2] K. Creath, “V Phase-Measurement Interferometry Techniques,” in Progress in Optics, vol. 26, Elsevier, 1988, pp. 349–393. doi: 10.1016/S0079-6638(08)70178-1.

[3] Y. Park, C. Depeursinge, and G. Popescu, “Quantitative phase imaging in biomedicine,” Nat. Photonics, vol. 12, no. 10, Art. no. 10, Oct. 2018, doi: 10.1038/s41566-018-0253-x.

Acknowledgement:
This research was funded by grants of the MCID and UEFISCDI, specifically projects PN-III-P2-2.1-PED-2016-0446, PN-III-P2-2.1-PED-2019-1264, and Program-Core research-development, ctr. no. 16N /08.02.2019. Gabriel Popescu was supported by NIH, through R01GM129709 and R01CA238191; R43GM133280-01.