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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-24 11:20 |
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Conference: Bucharest University Faculty of Physics 2021 Meeting
Section: Biophysics; Medical Physics
Title:
The interaction of BSA and levothyroxine with vitamins C, B12 and folic acid
Authors:
Ana-Maria IGNAT, Nicoleta SANDU, Claudia CHILOM
*
Affiliation:
Department of Electricity, Solid Physics and Biophysics, Faculty of Physics, University of Bucharest, Măgurele, Romania,
E-mail
ana_ignat99@yahoo.com
Keywords:
BSA, vitamins, levothyroxine, competitive binding, protein stability
Abstract:
Bovine serum albumin (BSA) is a globular protein with many significant properties such as transport, antioxidant and anti-inflammatory functions. The structure and the stability of BSA depend of pH value, temperature or antioxidants such as vitamins. Furthermore, due to its ability to improve the results of patients with cirrhosis, BSA is considered a niche medicine and it is used in manufacturing process of the biopharmaceuticals.
The most recommended treatment for thyroid dysfunctions is levothyroxine (LT4), the synthetic form of the thyroid hormone T4 [1]. Thus, in case of hyperthyroidism, LT4 is suppressing the concentration of hormones produced by the thyroid gland. On the other hand, when a patient suffers of hypothyroidism, LT4 is used as a replacing hormone.
The vitamins are organic compounds which can be classified as water soluble vitamins and fat soluble vitamins. One of the best known and used water soluble vitamins is the vitamin C, which has an essential role in formation of tendons and skin. Vitamin B12 is an important factor in the production of erythrocytes and performs important roles at the cellular level. Folic acid is an essential vitamin for cell growth and it is responsible for the DNA synthesis, being a recommended treatment in pregnancy. The structure of the BSA protein is influenced by the action of the vitamins and thyroid hormone T4 [2].
The aim of this study was to determine the stability of BSA protein in terms of structure as well as to present the binding mechanism of the vitamins C, B12 and folic acid to the BSA-LT4 complex. By using fluorescence methods, important parameters have been determined – the strength of the interactions and the thermodynamic parameters - enthalpy and entropy variation along with the Gibbs free energy variation.
References:
[1] S. Benvenga, A. Carlé, Levothyroxine Formulations: Pharmacological and Clinical Implications of Generic Substitution, Advances in Therapy, 36, 59-71, 2019 doi:10.1007/s12325-019-01079-1
[2]. N. Sandu, C. G. Chilom, M. David, M. Florescu, Evaluation of the interaction of levothyroxine with bovine serum albumin using spectroscopic and molecular docking studies, Journal of Biomolecular Structure and Dynamics, 1-13, 2020 https://doi.org/10.1080/07391102.2020.182291
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