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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 2:30 |
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Conference: Bucharest University Faculty of Physics 2021 Meeting
Section: Polymer Physics
Title:
Polymer-proteins-based coatings characteristics influence on cells behavior
Authors:
Madalina ICRIVERZI(1, 2), Laurentiu RUSEN(3), Anca BONCIU(3,4), Valentina MARASCU(3,5), Anca ROSEANU(1) and Valentina DINCA(3)
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Affiliation:
1) Institute of Biochemistry of the Romanian Academy, Bucharest, Romania;
2) Department of Biochemistry and Molecular Biology,University of Bucharest, Faculty of Biology, 91-95 Splaiul Independentei, 050095, Bucharest, Romania;
3) National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor, 077125, Magurele, Romania; * FOTOPLASMAT center;
4) Faculty of Physics, University of Bucharest,RO-077125, Magurele, Romania;
5) Université Paris-Saclay, CEA, INRAE, DMTS, SCBM, F-91191 Gif-sur-Yvette, France
E-mail
valentina.dinca@inflpr.ro
Keywords:
mesenchymal stem cells, oteogenic differentiation, lactoferrin, polymer composite
Abstract:
The potential of Mesenchymal stem cells (MSC) for implantology and cell-based therapy represents one of the ongoing research subjects within the last decades. In bone regeneration applications, the various environmental factors including bioactive compounds such growth factors, chemicals and physical characteristics of biointerfaces are the key factors in controlling and regulating osteogenic differentiation from MSCs. In our study we have investigated the influence of Lactoferrin (Lf) and Hydroxyapatite (HA) embedded within a biodegradable copolymeric matrix on the osteogenic fate of MSCs. The complex composite coatings based on biodegradable PEG-PCL copolymer and the two bioactive factors, Lf and HA were created via a laser evaporation technique. Scanning Electron Microscopy, EDAX, contact angle and surface energy of the analyzed coatings were correlated to biological response on both short and longer term (72h, respectively 28 days). Human MSC were cultured on the developed coatings and viability, proliferation and morphology were evaluated. All surfaces were shown not to exhibit toxicity, as confirmed by LIVE/DEAD assay. Lf-HA composite exhibited an increase in osteogenic differentiation of hMSC cells, results supported by ALP and mineralization assays. This is the first report about biodegradable composite layers directing osteogenic differentiation from hMSCs and the results indicated that the biodegradable layers have great potential for application in bone regeneration.
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