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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 1:42 |
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Conference: Bucharest University Faculty of Physics 2021 Meeting
Section: Polymer Physics
Title:
Preparation and characterization of cerium doped hydroxyapatite thin films
Authors:
Alina PRODAN (1,2), Mircea BEURAN (1,2), Simona Liliana ICONARU (3), Carmen Steluta CIOBANU (3), Carmen CHIFIRIUC (4,5,6), Luminita MARUNTESCU (4,5), Marcela BUCUR (5), George A. STANCIU (7), Radu HRISTU (7), Anton FICAI (8,9,10), Bogdan Stefan VASILE (8,11), Ionela Andreea NEACSU (8,11), Ecaterina ANDRONESCU (8), Roxana TRUSCA (8), Daniela PREDOI (3)
Affiliation:
1) Department of Surgery, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari, Sector 5, 050474 Bucharest, Romania
2) Emergency Hospital Floreasca Bucharest, 8 Calea Floresca, 014461 Bucharest, Romania
3) Multifunctional Materials and Structures Laboratory, National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125 Magurele, Romania;
4) Life, Environmental and Earth Sciences Division, Research Institute of the University of Bucharest (ICUB),University of Bucharest, 060023 Bucharest, Romania
5) Microbiology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane,
77206 Bucharest, Romania
6) Academy of Romanian Scientists, Ilfov Street, No. 3, 50044 Bucharest, Romania
7) Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania;
8) Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, University Politehnica of Bucharest, Bucharest, Romania
9) National Centre for Micro- and Nanomaterials, University POLITEHNICA of Bucharest, 060042 Bucharest, Romania
10) Academy of Romanian Scientists, 060042 Bucharest, Romania
11) National Research Center for Micro and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 060042 Bucharest, Romania
E-mail
dpredoi@gmail.com
Keywords:
cerium doped hydroxyapatite, thin films, antimicrobial properties, Confocal laser scanning microscopy
Abstract:
Recently, scientific research has focused on the development of new drug delivery systems. Hydroxyapatite [HAp, Ca10 (PO4) 6 (OH) 2] is a well-known bioceramic material that has been widely used as a bone substitute due to its suitable mechanical properties and similar to bone mineral composition. Improving the properties of HAp can be obtained by doping it with various ions. Therefore, obtaining thin layers of cerium doped HAp is of great interest for the biomedical field. The cerium doped hydroxyapatite (xCe = 0.1) thin films on Si substrate were obtained by spin coating method. The HApCe thin films were tested using scanning electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. The surface of HApCe_1 thin films was homogenous and continuous with no evidence of cracks. The elemental mapping results shows a uniform distribution of the constituent elements on the sample surface. The FTIR spectra together with the Raman spectra highlight the presence of vibrational bands corresponding to the phosphate and hydroxyl groups specific to the HAp structure. In both spectra, no additional vibrational bands were noticed. The antimicrobial assays proved that HApCe_1 thin films strongly inhibited the development of C. albicans fungal strains compared to control. Confocal laser scanning microscopy (CLSM) was performed to assess the fungal biofilm development on the surface of HApCe_1 thin films, after 24 h of incubation, by visualization of the adherent fungal cells on the substrates. CLSM images emphasized that the studied samples strongly inhibited the development of C. albicans fungal cells. Morphological and optical characterizations showed that hydroxyapatite doping was performed efficiently with cerium ions thus obtaining thin layers with superior antimicrobial properties.
Acknowledgement:
This research was funded by the Romanian Ministry of Research and Innovation through the project PN-III-P1-1.2-PCCDI-2017-0629/contract no. 43PCCDI/2018.
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