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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-24 11:30 |
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Conference: Bucharest University Faculty of Physics 2021 Meeting
Section: Biophysics; Medical Physics
Title:
Artificial lipid membranes: applications in cancer therapy
Authors:
Mădălina-Georgiana CHIVU (1),
Marcela-Elisabeta BĂRBÎNȚĂ-PĂTRAȘCU (1),
Mihaela BACALUM (2)
Affiliation:
1) University of Bucharest, Faculty of Physics, Department of Electricity, Solid-State Physics and Biophysics
2) Horia Hulubei National, Institute for Physics and Nuclear Engineering, Department of Life and Environmental Physics
E-mail
chivugeorgiana79@yahoo.com
Keywords:
liposomes, folic acid, silver nanoparticles
Abstract:
It is well known that cancer is a vast clinical and research domain being very interesting one in the scientific fields. This disease is in the attention of the researchers and physicians, in order to find new methods for therapy. To achieve this goal, researchers are turning their attention to the world of nanotechnology. In the last decade, metal nanoparticles have been shown to have a significant effect in improving the specific targeting of drugs in cancer therapy.
In this study, folic acid-functionalized silver nanoparticles (FA-AgNPs) have been shown to be effective against HT-29 colon cancer tumor cells, in a concentration-dependent manner. Thus, for FA-AgNPs concentrations higher than 0.71 µM, the viability of HT-29 cells decreased drastically. These metallic nanoparticles can be used as therapeutic agent delivery systems, being useful for the development of an alternative treatment strategy for cancer therapy in the near future.
Chlorophyll a (Chla) was used as an optical sensor to monitor in vitro, by UV-Vis absorption spectroscopy, the effect of FA-AgNPs on artificial cell membranes. Chla detected the insertion of FA-AgNPs into artificial lipid membranes. Thus, as the concentration of FA-AgNPs increased, the spectral signature of absorbance of Chla in the biomimetic membranes changed. This can be explained by generation of an energy transfer or electron transfer process when the porphyrin ring of Chla located at interface of the lipid bilayer/aqueous medium interacts with the FA-AgNPs surface.
Folic acid-functionalized AgNPs can be used in the development of liposome-based biohybrids, which can be used in the treatment of colorectal cancer.
Acknowledgement:
M.E.B.-P. acknowledges the funding through Projects JINR–RO 2021 No. 38/2021 and No. 68/2021 (IUCN Order 365/11.05.2021)
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