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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-24 11:56 |
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Conference: Bucharest University Faculty of Physics 2021 Meeting
Section: Biophysics; Medical Physics
Title:
Magnetic resonance methods in the study of the effects of ionizing radiation on biological systems
Authors:
Ioana FIDEL (1,2), Diana SERAFIN (1,2) Florin TELEANU (2), Silvana VASILCA (2), Roxana POPESCU (2), Andi CUCOANEȘ (2), Paul VASOS (1,2), Vasile BERCU (1)
*
Affiliation:
1) University of Bucharest
2) Extreme Light Infrastructure - Nuclear Physics
E-mail
fidel_ioana@yahoo.com
Keywords:
ionizing radiation, free radicals, spin-trap, electron paramagnetic resonance
Abstract:
Laser radiation can accelerate particles, which in turn act as ionizing radiation. This type of radiation is similar to that produced by particle accelerators. When interacting with matter, a series of reactive molecular species are generated such as the hydroxyl radical (*OH) or the superoxide anion (*O2-)that play a negative role in cell development. These free radicals are paramagnetic species that have an unpaired electron on the valence layer. A significant increase in free radical concentration will lead to alteration and malfunction of nucleic acids, enzymes or proteins, also changing the concentrations and kinetics of metabolites inside cells. Investigation of water radiolysis may provide representative results for investigating the interaction of radiation with matter because water is found in large amounts in the body [1] and studies show that most ionization due to interaction with radiation is caused by contact with water [2]. The spin-trapping technique involves the capture of free radicals (which have a short lifespan) by a spin trap that leads to the formation of a longer-lived adduct (molecular species that resist for minutes or longer in solution at ambient temperatures). Paramagnetic electron resonance spectroscopy is used to detect the presence of unpaired electrons in biological samples [3]. The experiments we have conducted have the role of preparing the future experiments that will be performed with the help of high-power lasers. Following irradiation with protons or electrons of water samples with BMPO, at different dose rate, a series of EPR analyzes were performed in order to obtain information about the type and quantity of free radicals produced. We comment in this work on the observed quantities of free radicals and draw preliminary conclusions about the possibility of following by magnetic resonance the effect of radiation with different dose rates on living systems.
References:
1 B.G. Ershov, A.V. Gordeev / Radiation Physics and Chemistry 77, 928–935, 2008
2 D. Grosch, Biological Effects of Radiations, Academic Press, 1979
3 J.Y. Ren et al., Journal of Magnetic Resonance, 166, 82–91, 2004
Acknowledgement:
The authors thank Cezara Zăgrean-Tuza for assistance with EPR analysis and
for useful discussions.
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