UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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2024-11-23 18:29
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Conference: Bucharest University Faculty of Physics 2022 Meeting


Section: Biophysics; Medical Physics


Title:
The influence of synthetic antimicrobial peptide P8 on the membrane fluidity using laurdan fluorescence


Authors:
Francesca-Giulia GUARNERI (1), Marcela-Elisabeta BĂRBÎNȚĂ-PĂTRAȘCU (1), Mihaela BACALUM (2)


Affiliation:
1) University of Bucharest, Faculty of Physics, Department of Electricity, Solid-State Physics and Biophysics, Atomistilor Street 405, 077125 Măgurele, Ilfov, Romania;

2) Horia Hulubei National Institute for Physics and Nuclear Engineering, Department of Life and Environmental Physics, Reactorului 30, 077125 Măgurele, Ilfov Romania;


E-mail
giuliafrancesca22@gmail.com


Keywords:
antimicrobial peptides, laurdan, membrane fluidity


Abstract:
Antimicrobial peptides (AMPs) are an important class of short peptides that can be found in humans, animals, and plants with a wide spectrum of antibacterial, antifungal, antiparasitic, and antiviral properties [1], [2]. We conducted a study regarding the influence of the synthetic antimicrobial peptide P8 (HRWWRWWRH-NH2) on the membrane fluidity of two different cell lines (BJ and B16) using the General Polarization (GP) values, a key parameter to obtain important information on the behaviour of the mentioned membranes. Laurdan, which is known to sense the polarity changes in the environment, was used as a fluorescent membrane probe to label the BJ and B16 cells, the first being healthy skin cells and the second the melanoma cell line. P8 was added in varying concentrations. The temperature was increased from 20°C to 37°C to further investigate the peptide’s effect on the cells. The results showed significant changes induced by the AMPS on the membrane fluidity of both cell lines, confirming that the experimental protocol was efficient in assessing the desired parameter. This protocol will be further developed to better understand the complex interactions that occur between AMPs and the cellular membranes.


References:

[1]. Li, J., Hu, S., Jian, W. et al., Plant antimicrobial peptides: structures, functions, and applications. Bot. Stud. 62, 5 (2021). https://doi.org/10.1186/s40529-021-00312-x

[2]. Huan Y, Kong Q, Mou H and Yi H (2020), Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields. Front. Microbiol. 11:582779. doi:10.3389/fmicb.2020