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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-23 18:30 |
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Conference: Bucharest University Faculty of Physics 2022 Meeting
Section: Physics and Technology of Renewable and Alternative Energy Sources
Title:
Antioxidant potential of Hippophae rhamnoides L. extracts
Authors:
Ana Lăcrămioara APETREI(1), Cornelia DIAC(1), Sanda VOINEA(1,2), Cornelia NICHITA(1,2)
Affiliation:
1. University of Bucharest, Faculty of Physics, CTT 3Nano-SAE Research Center, MG-38, 405 Atomistilor Street, 077125, Magurele, Romania, cornelia.nichita@unibuc.ro, cornelia@3nanosae.org
2. National Institute for Chemical – Pharmaceutical Research and Development, 112 Vitan Avenue, 031299, Bucharest, Romania,
E-mail
cornelia.nichita@unibuc.ro, cornelia@3nanosae.org
Keywords:
Hippophae rhamnoides L. extracts, phenolic compounds, antioxidant potential
Abstract:
Hippophae rhamnoides L. belongs to the Elaeagnaceae family and is an extremely valuable plant source with a complex chemical composition rich in multiple vitamins (A, B1 , B12, C, E, K, and P) carotenoids (lutein, B-carotene, zeaxanthin and lycopene) organic acids and micro-macro nutrients , phytosterols, fatty acids, such as gamma-linolenic acid (omega-6) and palmitooleic acid (omega-7), proteins, carbohydrates, trace elements (Fe, Zn, Ca, Mg, Se) and phenolic compounds (flavonoids, polyphenolic acids). Various parts of Hippophae rhamnoides L , particularly berries possess various pharmacological activities, such as antimicrobial, anti-inflammatory, immunomodulatory, antioxidant, and dermatological effects, generated by the complexity of the chemical composition mentioned above. In present study, the aim is to explore the influence of the concentration of the extraction solvent (used in the Soxhlet method) on the content of phenolic compounds and the antioxidant potential, as well as the relationship between them. By UV-VIS (Jasco, Japan, V-570 spectrophotometer) spectrometric method were determined: total polyphenolic contents (TPC) expressed as gallic acid equivalent/g (mg/GAE g-1) using the Folin-Ciocalteu reagent, total flavonoid contents (TFC) expressed as rutin equivalent/g (mg/RE g-1), by aluminum chloride colorimetric assay, caffeic acid derivatives content (CADC) expressed as mg caffeic acid equivalent/g dry extract (mg/CAg-1) by using Arnows’ reagent in according to the procedure described in European Pharmacopoeia 6th edition. The antioxidant potential were investigated in vitro non cellular assays, by DPPH(2,2-diphenyl-1-picrylhydrazyl) free radical scavenging, and chemiluminescence technıque. All methods of investigation highlighted that: i) increasing the concentration of the extraction solvent leads to a higher amount of extracted phenolic compounds ii) the antioxidant potential and the content of phenolic compounds are linear correlated.
Acknowledgement:
The support from the CTT 3 Nano-SAE Research Centre, Faculty of Physics, University of Bucharest, are thankfully acknowledged.
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