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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-23 18:32 |
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Conference: Bucharest University Faculty of Physics 2022 Meeting
Section: Polymer Physics
Title:
Development and characterisation of novel ZnO/hydroxyapatite/chitosan composites for wound treatments
Authors:
Steluta Carmen CIOBANU (1), Stefania Mariana RAITA (2), Gabriel PREDOI (2), Coralia BLEOTU (3,4), Simona Liliana ICONARU (1), Daniela PREDOI (1)
Affiliation:
1) Multifunctional Materials and Structures Laboratory, National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125 Magurele, Romania; simonaiconaru@gmail.com (S.L.I.); ciobanucs@gmail.com (C.S.C.); dpredoi@gmail.com (D.P.)
2) Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 105 SplaiulIndependentei, Sector 5, 050097 Bucharest, Romania;gabrielpredoi2017@gmail.com (G.P.); raitastefania@gmail.com (S.M.R.);
3) Stefan Nicolau Virology Institute, 285 Mihai Bravu Avenue, Sector 3, Zip Code 030304, PO 77, PO Box 201, Bucharest, Romania, coralia.bleotu@virology.ro (C.B.);
4) Research Institute of the University of Bucharest (ICUB), Life, Environmental and Earth Sciences Division, University of Bucharest, 060023 Bucharest, Romania
E-mail
dpredoi@gmail.com
Keywords:
Abstract:
The aim of this study was to obtain new ZnO/hydroxyapatite/chitosan (ZHC) compositesand to study their physico-chemical and biological properties. The biological properties were evaluated by in vitro and in vivo assays. The antifungal activity of ZHC composite against Candida albicans ATCC 10231 fungal strain was also studied. On the other hand, complex physico-chemical characterisations of the obtained composites was performed using methods such as: Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The ZHC composite were obtained by an adapted coprecipitation method. The SEM studies revealed that the obtained samples consist in nanometric particles with arod-like morphology. Energy dispersive X-ray (EDX)spectra highlighted the presence of Zn, O, Ca, P and N in the ZHC composite. Moreover, the presence of chitosan on the surface of the ZHC was sustained by the results of the FTIR studies. Also, the presence ofZn–O bond at 467 and 415 cm−1 was noticed in the FTIR spectra.The results of antifungal assay proved that the ZHCS composites inhibited C. albicans biofilm formation having an excellent inhibitory effect against the development of C. albicansmicrobial cells. The cytotoxicity of ZHC composite was evaluated using human osteosarcoma MG63 (ATCC CRL 1427) cell line. The results of the MTT assay, suggest that the ZHC possess a good biocompatibility and did not induce any harmful effect on the studied cells. The results of the in vivo test conducted on the rats with artificial induced wounds and treated with ZHC composite, suggest that our composite promote their accelerated healing. Therefore, our results indicate that the novel ZnO/hydroxyapatite/chitosan compositesmay represent a starting point for the development of new innovative wound treatments.
Acknowledgement:
This work was supported by the Romanian Ministry of Research and Innovation through the project PN-III-P2-2.1-PED-2019-1375, contract number contract number 331PED⁄2020.
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