UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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2024-11-23 18:11
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Conference: Bucharest University Faculty of Physics 2023 Meeting


Section: Polymer Physics


Title:
MAPLE Modified Polydimethylsiloxane (PDMS) Interfaces Characteristics Influence on Cell Behavior


Authors:
Madalina ICRIVERZI(1), Paula FLORIAN(1), Anca BONCIU(2), Nicoleta DUMITRESCU(2), Laurentiu RUSEN(2), Anca ROSEANU(1), Valentina MARASCU(2), Valentina DINCA(2)


Affiliation:
1) Institute for Biochemistry of Romanian Academy, 060031 Bucharest, Romania

2) National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania



E-mail
valentina.dinca@inflpr.ro


Keywords:
MAPLE; fibroblasts; biodevices


Abstract:
One strategy to develop new and more efficient biodevices used as implants is based on surface physical-chemical modifications in order to prevent undesired biological responses [1, 2]. The physical-chemical characteristics of the newly proposed Poly(2-methacryloyloxyethyl phosphorylcholine) (pMPC) functionalized scaffold obtained by Matrix-Assisted Laser Evaporation (MAPLE) method [3] were evaluated by Scanning Electron Microscopy, Atomic Force Microscopy, Contact angle, Surface energy, Fourier Transform Infrared Spectroscopy and X-ray Photoelectron Spectroscopy. The assessment of adhesion, proliferation and morphology of cells grown on the functionalized Polydimethylsiloxane (PDMS) surfaces was performed in vitro, using human macrophages and fibroblasts, cells involved in foreign body reaction. The results obtained after evaluation of the physical–chemical properties of the new coatings revealed that the MAPLE technique proposed has the advantage of achieving homogeneous, stable and moderate hydrophilic thin layers onto hydrophobic PDMS. Moreover, this approach does not require any pre-treatment, therefore avoiding the major disadvantage of hydrophobicity recovery. Biological investigation evidenced the reduction of the adhesion and proliferation of human macrophages by ~50% and of human fibroblast by ~40% on the modified surfaces of PDMS compared to unmodified scaffold, thus circumventing undesired cell responses such as inflammation and fibrosis. All these highlighted the potential for the new PDMS interfaces obtained by MAPLE to be used in the biomedical field to design PDMS-based implants exhibiting long-term hydrophilic profile stability and better mitigating foreign body response.


References:

[1]. J.R Smith, D.A. Lamprou, Trans. IMF 2014, 92, 9–19.

[2]. Melo, F. J.Crijns, Hosp. Infect. 2018, 99, 239–249.

[3]. M. Icriverzi, L. Rusen, S. Brajnicov, A. Bonciu, M. Dinescu, A. Cimpean, R.W.Evans, V. Dinca, A. Roseanu, Coatings 2019, 9, 236 https://doi.org/10.3390/coatings9040236.



Acknowledgement:
This research was funded by a grant of the Romanian Ministry of Education and Research, project number project PN-III-P4-ID-PCE-2020-2375. P.F., M.I. and A.R. acknowledge and thank the partial support of Project No.5 of the Structural and Functional Proteomics Research Research Program of the Institute of Biochemistry of the Romanian Academy.