Faculty of Physics - University of Bucharest

UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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2024-11-23 18:30

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Conference: Bucharest University Faculty of Physics 2023 Meeting

Section: Biophysics; Medical Physics

Title:

Magneric resonance biomarkers for timely detection of high dose-rate radiation effects in cells

Authors:

Diana SERAFIN(1,2), P. R. VASOS(1,3), C. ZĂGREAN-TUZA(1,3,4), I. FIDEL(1,3), S. VASILCA(1,3), D. NEGUȚ(1), M. SUDITU(5), R. POPESCU(1,6), M. BACALUM(1), G. GIUBEG (1), L. NEAGU(1), P.G. BLEOTU (1), M. ROȘU(1), E. HERMANN(3), M. MIREA(3), L. NIȚĂ 6), M. IOVEA(7), O. TEȘILEANU(1), A. HANGANU(3), M. A. VODĂ(1), A. SADET(1), and I.C. CHIRICUȚĂ(5)

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Affiliation:

1)Extreme Light Infrastructure - Nuclear Physics (ELI-NP), Biophysics and Biomedical Applications Laboratory and Group, Laser Gamma Experiments Department, LGED, “Horia Hulubei” National Institute for Physics and Nuclear Engineering (IFIN-HH), Life Sciences and IRASM Departments – Radiation Processing Centre, 30 Reactorului Street, RO-077125 Bucharest-Magurele, Romania

2)University of Bucharest, Faculty of Physics, 405 Atomistilor Street, P.O. Box MG-11, 077125 Magurele, Romania

3)Interdisciplinary School of Doctoral Studies, ISDS, and Departments of Physics and Chemistry, University of Bucharest, B-dul Regina Elisabeta, Bucharest, Romania

4)Universitatea Babes-Bolyai, Strada Mihail Kogălniceanu 1, Cluj-Napoca 400347, Romania

5)Amethyst Radiotherapy, Șoseaua Odăii 42, Otopeni 075100, Romania

6)Politehnica University of Bucharest, Faculty of Medical Engineering, Department of Bioengineering and Biotechnology, Str. Polizu 1-7, Bucharest, RO- 011061, Romania

7)AccentPro2000 S.R.L (AP2K), 4 Marasesti Str., RO 077125, Magurele-ILFOV, Romania

E-mail

dcserafin10@yahoo.com

Keywords:

Abstract:

In the recent use of radiotherapy to treat and control various types of cancer, dose-rates are explored to minimize radiation toxicity. Molecular biomarkers evaluated via in-cell studies have the potential of monitoring the radiation dose and dose-rate response within a biologically relevant time-window of up to tens of hours. We investigate the metabolic changes induced by radiation in cells via Magnetic Resonance Spectroscopy. The presentation will detail procedures used for metabolic imaging of radiation effects in glioblastoma U251 MG cell cultures. The specific investigation of metabolic pathways using carbon-13 isotopically-enriched glucose will be discussed. When it comes to profiling metabolites using nuclear magnetic resonance (NMR) spectroscopy, there are typically two significant challenges that need to be addressed: the requirement to mitigate interference caused by the substantial concentration of large molecules and the need for the identification and quantification of individual metabolites. This presentation will contain two methods for the alleviation of interference from proteins and other molecules, using magnetic resonance size-dependent parameters and, respectively, filters.

References:

(1) M-C Vozenin, J. H. Hendry , C.L. Limoli, Biological Benefits of Ultra-high Dose Rate FLASH Radiotherapy: Sleepy Beauty Awoken

https://pubmed.ncbi.nlm.nih.gov/31010708/

(2) Undurti N. Das, Molecular Biochemical Apects of Cancer