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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-23 17:56 |
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Conference: Bucharest University Faculty of Physics 2023 Meeting
Section: Physics and Technology of Renewable and Alternative Energy Sources
Title:
Free radical scavenging properties of N-acetylcysteine (NAC)
Authors:
Cornelia NICHITA(1,3), Marcela Elisabeta BĂRBINŢA-PĂTRAŞCU(2)
Affiliation:
1) University of Bucharest, ICUB, Faculty of Physics, CTT-3Nano-SAE Research Center, MG-38, 405 Atomistilor Street, 077125, Magurele, Romania
2) University of Bucharest, Faculty of Physics, Department of Electricity, Solid-State Physics and Biophysics, 405 Atomistilor Street, PO Box MG-11, Bucharest-Magurele, 077125, Romania
3) National Institute for Chemical – Pharmaceutical Research and Development, 112 Vitan Avenue, 031299, Bucharest, Romania
E-mail
cornelia.nichita@unibuc.ro
Keywords:
N-acetylcysteine, radical scavenging, antioxidant activity, chemiluminescence technique
Abstract:
N-acetylcysteine (NAC) is a derivative of the amino acid cysteine and is a precursor to glutathione, an important antioxidant in the body. NAC has been shown to have free radical scavenging properties, which means it can help protect cells from damage caused by reactive oxygen species (ROS) and other harmful molecules. The complex pharmacological action of N-acetylcysteine (NAC) allows its use in the treatment of various diseases such as liver failure, inflammatory processes but also nephropathy and brain disorders. N-acetylcysteine (NAC) presents a therapeutic efficacy due to its ability to reduce extracellular cystine to cysteine, and as a source of sulfhydryl groups. NAC stimulates glutathione synthesis, enhances glutathione-S-transferase activity, promotes liver detoxification by inhibiting xenobiotic biotransformation, and is a powerful nucleophile capable of scavenging free radicals. Starting from these considerations, the present paper aims to evaluate the scavenger effect of N-acetylcysteine by three distinct methods. Thus, the scavenger effect of free radicals was assessed by employing in vitro non cellular assays using the chemiluminescence technique in aminophthalhydrazide-hydrogen peroxide system at pH=8.6, DPPH(2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay and ABTS (2,2′azinobis-(3-ethylbenzthiazoline-6- sulfonic acid) methods. The results confirmed a significant value of antioxidant activity but also a good correlation between the three techniques approached.
Acknowledgement:
The support from the CTT 3 Nano-SAE Research Centre and the support from the Materials and Devices are thankfully acknowledged.
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