UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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2024-11-22 2:10
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Conference: Bucharest University Faculty of Physics 2010 Meeting


Section: Applied Nuclear Physics


Title:
The purification and the quality control of 68Ga eluate


Authors:
I. Patrascu (1), D. Niculae (1), V. Lungu (1), I. Ursu (1), M. Iliescu (1), C. Tuta (1), A. Antohe (1)


Affiliation:
(1) Horia Hulubei National Institute for Physics and Nuclear Engineering


E-mail
ipatrascu@nipne.ro


Keywords:
68Ge/68Ga generator, purification, quality control, HPLC


Abstract:
Today the majority of PET studies are performed with F-18 radiopharmaceuticals requiring an on-site cyclotron or shipment from a site in close proximity to the place where the investigation is performed. Generator based radionuclides would allow easier availability and more flexibility in use. 68Ge/68Ga generators provide cyclotron-independent access to positron emission tomography (PET) radiopharmaceuticals. 68Ga is a short-lived positron emitter (half-life 67.6 min) and is coming from his parent 68Ge, which have a half-life of 270.8 days. The 270-days half-life of the parent allows the use of the generator for a long period, potentially up to 1 year or even longer. The 67.6 min half-life of 68Ga matches the pharmacokinetics of many peptides and other small molecules owing to rapid diffusion, localization at the target and fast blood clearance.The 68Ga solutions eluted from the generator are usually containing small amounts of other cations. Before the radiolabelling of peptides with it, we have to purify the eluate. Because of the metallic impurities (breakthrough of Ge, Fe, Zn), the eluate has to be purified either on a cation exchange column, on a anion exchange column or both of them, combined. In this paper it will be presented the results obtained after the purification on a cation exchange column, anion exchange column, both of them and fractionated elution. Purification using both resins and fractionated elution are feasible leading to a high quality eluate suitable for peptides radiolabelling.