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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-22 1:38 |
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Conference: Bucharest University Faculty of Physics 2013 Meeting
Section: Biophysics; Medical Physics
Title:
Microwave genotoxic impact and their influence on oxidative stress in young seedlings
Authors:
Diana Vrincianu (1), Claudia Nadejde (1), Dorina Creanga (1), Florin Tufescu (1), Gabriela Vochita (2)
Affiliation:
(1)”Al.I.Cuza” University, Physics Faculty, Iasi, Romania
(2)Institute of Biological Research, Iasi, Romania
E-mail
dianavrincianu@yahoo.com
Keywords:
power density microwaves, chromosomal aberration, peroxidasic activity
Abstract:
In this paper studies of genotoxicity and oxidative stress induced by microwave exposure in maize seedlings are presented. Biological material was consistent with uniform genophond caryopses provided by experimental micro-populations of Zea mays or common maize. Four uniform vegetal samples cultivated on porous paper support were exposed to low power density microwaves during ten days for 1, 2, 4 or 12 hours daily. The cytogenetic analyses were carried out on embryos tissues prevailed from freshly germinated root meristems according to Feulgen method and squash technique. Cell nucleus investigation showed microwave significant influence on mitosis rate as well as considerable effect on the aberrant cell divisions. The most of chromosomal aberrations were identified in anaphase for all sample arrays like: intrechromatidian bridges, expelled or retard chromosomes and chromosome fragments, micronuclei and complex aberrations as combinations of those. The increased oxidative stress level was evidenced by enhanced activity of peroxidase like enzymes. Data interpretation was based on the cell metabolism adaptation to the environmental constraint – consistent with peroxidase biosynthesis stimulation that also could overlap onto the enzyme inactivation effect especially for long time exposures to microwaves. Non-thermal interaction of microwaves with vegetal cells of young seedlings during their early ontogenetic stages may represent the main phenomena underlying the observed effects.
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