| |
 |
UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-24 21:14 |
 |
|
|
|
Conference: Bucharest University Faculty of Physics 2001 Meeting
Section: Biophysics; Medical Physics
Title:
The Laser Effect on Mycoplasma Agalactiae
Authors:
Cerasela Z. Dinu, Constantin Grigoriu, Maria Dinescu,
*Florentina Pascale, *Andrei Popovici, *Lavinia Gheorghescu, *Ana Cismileanu
**Eugenia Avram
Affiliation:
National Institute for Lasers, Plasma and Radiation Physics, Bucharest
*National Society “Pasteur Institute” S.A.,
**"Spiru-Haret" University
E-mail
Keywords:
Abstract:
The effects of the laser irradiation on the bacteria from the species Mycoplasma agalactiae are reported.
These bacteria belong to Mycoplasma type, denominated Pleuropneumonia Like Organisms (PPLO). Mycoplasma agalactiae is a respiratory aviary mycoplasma, which produces contagious agalaxia of the sheep and goats. Their dimensions vary between 250 and 5000 nm. Their cultivation is made in colony and agar PPLO, containing specific nutrients.
In order to perform the laser irradiation, the bacteria, in colony medium or in tris-HCl buffer, were put in Petri dishes. The irradiation was done with a Nd: YAG laser working in visible range (green radiation, with the wavelength 532 nm). The cellular biological response to laser irradiation consists in physical and/or chemical modifications of the photoacceptors (chromophores), which are molecular components of the respiratory chain.
The electrophoresis and electron microscopy techniques were used in order to put evidence the structural and functional modifications induced by laser irradiation. The preliminary results indicate that a cell proliferation takes place in the mentioned experimental conditions.
|
|
|
|